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食品与发酵工业  2022, Vol. 48 Issue (15): 288-294    DOI: 10.13995/j.cnki.11-1802/ts.029880
  分析与检测 本期目录 | 过刊浏览 | 高级检索 |
UPLC/MSMS内标法测定火锅食品中5种生物碱和15种喹诺酮类物质
杨昌彪1, 李占彬1, 阙云飞2, 黄永桥2, 包娜1, 崔姗姗1, 赵莎2, 马凯1*
1(贵州省分析测试研究院,贵州 贵阳,550014)
2(贵州省检测技术研究应用中心,贵州 贵阳,550014)
Determination of 5 alkaloids and 15 quinolones in hotpot food by UPLC/MSMS with internal standard method
YANG Changbiao1, LI Zhanbin1, QUE Yunfei2, HUANG Yongqiao2, BAO Na1, CUI Shanshan1, ZHAO Sha2, MA Kai1*
1(Guizhou Academy of Analysis Test,Guiyang 550014, China)
2(Guizhou Testing Technology Research and Application Center, Guiyang 550014, China)
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摘要 该研究建立了UHPLC/MSMS内标法同时测定火锅食品中5种生物碱和15种喹诺酮类物质的分析方法。样品经1%甲酸乙腈沉淀蛋白,分散固相萃取净化后,在正离子ESI-MRM模式测定,内标法定量。药物组分在质量浓度1.0~250 ng/mL线性良好,相关系数≥0.997 5,方法检出限为0.5~5 μg/kg;方法平均回收率为74.6%~114.8%,相对标准偏差小于15.1%。该方法操作简单,灵敏度高,适合火锅食品中非法添加的喹诺酮类药物和生物碱检测确证。
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杨昌彪
李占彬
阙云飞
黄永桥
包娜
崔姗姗
赵莎
马凯
关键词:  超高效液相色谱-串联质谱  火锅食品  生物碱  喹诺酮类    
Abstract: The aim of this study was to establish a determination and analysis method for 5 alkaloids and 15 quinolones in hotpot food by UPLC/MSMS with the internal standard method. The sample was extracted by 1% formic acid acetonitrile, protein deposition and disperse solid-phase extraction purification, finally detected by positive ESI-MRM mode with the internal standard method. The result indicated that the standard curves of drug components were linear in the range of 1.0-250 ng/mL which had correlation coefficients greater than 0.997 5. The limits of detection were 0.5-5 μg/kg, the average recoveries of kinds of illegally added drugs were 74.6%-114.8% which with a relative standard deviation less than 15.1%. This method is simple, sensitive and suitable for the determination and confirmation of illegal quinolones and alkaloids in hotpot food.
Key words:  UPLC/MSMS    hotpot food    alkaloids    quinolones
收稿日期:  2021-11-03      修回日期:  2021-12-22           出版日期:  2022-08-15      发布日期:  2022-09-02      期的出版日期:  2022-08-15
基金资助: 中央引导地方科技发展资金(黔科中引地[2021]4026);贵州省高层次创新型“千”层次人才培养项目
作者简介:  第一作者:硕士,高级工程师(马凯高级工程师为通信作者,E-mail:472020603@qq.com)
引用本文:    
杨昌彪,李占彬,阙云飞,等. UPLC/MSMS内标法测定火锅食品中5种生物碱和15种喹诺酮类物质[J]. 食品与发酵工业, 2022, 48(15): 288-294.
YANG Changbiao,LI Zhanbin,QUE Yunfei,et al. Determination of 5 alkaloids and 15 quinolones in hotpot food by UPLC/MSMS with internal standard method[J]. Food and Fermentation Industries, 2022, 48(15): 288-294.
链接本文:  
http://sf1970.cnif.cn/CN/10.13995/j.cnki.11-1802/ts.029880  或          http://sf1970.cnif.cn/CN/Y2022/V48/I15/288
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