Abstract: Lactobacillus brevis was approved by the National Health Commission as a microbial strain for preparing γ-aminobutyric acid(GABA). An approach to produce GABA from L-glutamic acid using glutamic acid decarboxylase was developed. A strain of Lactobacillus brevis GLB-127 was identified by scanning electron microscopy and molecular biology, and a phylogenetic tree of this lactic acid bacteria was constructed. The preliminary technical route forms after optimization of the fermentation conditions and transformation conditions, including the culture rotate speed, culture temperature and the amount of whole cell catalytic enzyme. Then the fermentation medium was optimized made the glutamic acid decarboxylase activity and GABA output increases significantly. After magnifying culture in 10 L fermenter, the concentration of GABA reached 345.1 g/L, the conversion rate was 98.5%, and the enzyme activity of glutamic acid decarboxylase was 315.9 U/g. This research laid the foundation for the industrial production of GABA as a new food material.
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2022, Vol. 48 
