Abstract: Lycopene, a C40 terpenoid compound, has strong anti-oxidation and immunity enhancement effects, which have been widely used in the food and cosmetics industries. To improve the efficiency of heterologous production of lycopene, this study screened Escherichia coli top10 as the suitable production strain and optimized fermentation medium by single factor experiment and response surface methodology. The optimal medium was medium with 4.22 g/L fructose, 0.89 g/L NH4Cl and 15% n-dodecane. Under this condition, the yield of lycopene reached 29.3 mg/g DCW, which was 138% higher than before optimization. Based on real-time fluorescent quantitative PCR, the expressions of key genes IspG and crtE for lycopene synthesis were significantly enhanced (P<0.05) after optimization, which increased by 6.9 times and 4.5 times, respectively. This study significantly improved the yield of lycopene through host screening and medium optimization, laying the foundation for the efficient production of lycopene in E. coli.
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