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食品与发酵工业  2022, Vol. 48 Issue (15): 78-84    DOI: 10.13995/j.cnki.11-1802/ts.030565
  研究报告 本期目录 | 过刊浏览 | 高级检索 |
牛乳外泌体对动物双歧杆菌F1-3-2生长及益生特性的影响
周子寒, 刘琦琦, 郝海宁, 仝令君, 刘同杰, 公丕民, 张兰威, 易华西*
(中国海洋大学 食品科学与工程学院,山东 青岛,266000)
The effect of bovine milk exosomes on the growth and probiotic properties of Bifidobacterium animalis F1-3-2
ZHOU Zihan, LIU Qiqi, HAO Haining, TONG Lingjun, LIU Tongjie, GONG Pimin, ZHANG Lanwei, YI Huaxi*
(College of Food Science and Engineering, Ocean University of China, Qingdao 266000, China)
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摘要 采用超速离心法从牛乳中分离提取外泌体,通过免疫印迹法、纳米颗粒追踪技术、透射电镜对牛乳外泌体进行鉴定。评价了牛乳外泌体对动物双歧杆菌(Bifidobacterium animalis)F1-3-2生长及益生特性的影响。结果表明,牛乳外泌体促进了动物双歧杆菌F1-3-2的生长,提高了其耐酸、耐胆盐等肠胃环境耐受性,增加了菌体的黏附性。此外,牛乳外泌体增强了动物双歧杆菌F1-3-2的抗炎特性,炎症因子TNF-α、IL-1β水平显著下调。该研究为牛乳外泌体作为新型益生元的应用开发奠定了基础。
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周子寒
刘琦琦
郝海宁
仝令君
刘同杰
公丕民
张兰威
易华西
关键词:  牛乳  外泌体  双歧杆菌  生长  益生特性    
Abstract: Exosomes were extracted from bovine milk by ultracentrifugation. The bovine milk exosomes were identified by immunoblotting, nanoparticle tracking and electron microscopy after extraction. The effects of bovine milk exosomes on the growth and probiotic properties of Bifidobacterium animalis F1-3-2 were evaluated. The results showed that the bovine milk exosomes promoted the growth of B. animalis F1-3-2, improved the gastrointestinal tolerance of B. animalis F1-3-2, such as the acid resistance and the bile salt resistance. Besides, the adhesion of B. animalis F1-3-2 was also promoted. In addition, the bovine milk exosomes induced better anti-inflammatory properties of B. animalis F1-3-2, the levels of inflammatory cytokines like TNF-α, IL-1β significantly decreased. In conclusion, the bovine milk exosomes have the potential to be developed as a novel prebiotic.
Key words:  bovine milk    exosomes    Bifidobacterium    growth    probiotic properties
收稿日期:  2021-12-23      修回日期:  2022-01-11           出版日期:  2022-08-15      发布日期:  2022-09-02      期的出版日期:  2022-08-15
基金资助: 山东省自然科学基金重点项目(ZR2020KC009);国家自然科学基金面上项目(32172180)
作者简介:  第一作者:硕士研究生(易华西教授为通信作者,E-mail:yihx@ouc.edu.cn)
引用本文:    
周子寒,刘琦琦,郝海宁,等. 牛乳外泌体对动物双歧杆菌F1-3-2生长及益生特性的影响[J]. 食品与发酵工业, 2022, 48(15): 78-84.
ZHOU Zihan,LIU Qiqi,HAO Haining,et al. The effect of bovine milk exosomes on the growth and probiotic properties of Bifidobacterium animalis F1-3-2[J]. Food and Fermentation Industries, 2022, 48(15): 78-84.
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http://sf1970.cnif.cn/CN/10.13995/j.cnki.11-1802/ts.030565  或          http://sf1970.cnif.cn/CN/Y2022/V48/I15/78
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