Abstract: Exosomes were extracted from bovine milk by ultracentrifugation. The bovine milk exosomes were identified by immunoblotting, nanoparticle tracking and electron microscopy after extraction. The effects of bovine milk exosomes on the growth and probiotic properties of Bifidobacterium animalis F1-3-2 were evaluated. The results showed that the bovine milk exosomes promoted the growth of B. animalis F1-3-2, improved the gastrointestinal tolerance of B. animalis F1-3-2, such as the acid resistance and the bile salt resistance. Besides, the adhesion of B. animalis F1-3-2 was also promoted. In addition, the bovine milk exosomes induced better anti-inflammatory properties of B. animalis F1-3-2, the levels of inflammatory cytokines like TNF-α, IL-1β significantly decreased. In conclusion, the bovine milk exosomes have the potential to be developed as a novel prebiotic.
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