The simultaneous determination of flufiprole and its metabolites in soil-pakchoi system by QuEChERS-UPLC-MS/MS
LI Jing1,2,3*, DONG Chao1,2,3, ZUO Wei1,2,3, AN Wenjin1,2,3, ZHANG Yaohai1,2,3, ZHAO Qiyang1,2,3, JIAO Bining1,2,3*
1(Citrus Research Institute, Southwest University, Chongqing 400712, China)
2(Supervision＆Inspection＆Testing Center for Quality of Citrus Fruits＆Nursery Trees, Ministry of Agriculture and Rural Affairs, Chongqing 400712,China)
3(National Citrus Engineering Research Center, Chongqing 400712, China)
Abstract: A method for the analysis of flufiprole and its metabolites (flufiprole sulfone, flufiprole sulfide, flufiprole amide and fipronil) in soil-pakchoi (Brassica campestris L. ssp. chinensis) system was established using ultra-high performance liquid chromatography tandem mass spectrometry combined with QuEChERS pretreatment technology. The samples were rapidly extracted by acetonitrile and purified by multi-walled carbon nanotubes. Flufiprole and its metabolites were separated at a Titank F5 column (100×2.1 mm, 1.8 μm), detected with multiple reaction monitoring mode (MRM). Then, they were quantified with matrix matching standard curve. Excellent linear relationship of flufiprole and its metabolites in pakchoi samples (roots, stems and leaves) and soil samples were obtained in the concentration range of 0.5-100 μg/L with the correlation coefficient no less than 0.992 3. The limit of quantification (LOQ) was 5 μg/kg. The average recoveries of flufiprole and its metabolites in pakchoi samples (roots, stems and leaves) and soil samples were 76%-107%, and the relative standard deviation (RSD) was 0.8%-7.6%. The established method is suitable for the simultaneous analysis of flufiprole and its metabolites in soil-pakchoi system.
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LI Jing,DONG Chao,ZUO Wei,et al. The simultaneous determination of flufiprole and its metabolites in soil-pakchoi system by QuEChERS-UPLC-MS/MS[J]. Food and Fermentation Industries, 2022, 48(15): 273-280.
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2022, Vol. 48 
