Preparation and characterization of oat digestive resistant peptide nanocarrier and its embedding effect on caffeic acid phenethyl ester
JIN Zulong1, FENG Siyi1, HU Yawen1, SONG Hongdong1, GUAN Xiao1*, SUN Zhu2, DAI Zhihua3
1(School of Health Science and Engineering/National Grain Industry (Urban Grain and Oil Security)Technology Innovation Center, University of Shanghai for Science and Technology, Shanghai 200093, China) 2(Inner Mongolia Yangufang Ecological Agriculture Technology (Group) Co. Ltd., Hohhot 011700, China) 3(MDO Smarter Grain Technology Co. Ltd., Shanghai 200241, China)
Abstract: In order to improve the water solubility of oat protein and its application value in the field of nano materials, oat digestion resistant peptide nanoparticles were prepared by enzymatic hydrolysis, and their ability to encapsulate hydrophobic active substances and digestion resistance were evaluated. Oat protein was digested by pepsin and trypsin to prepare oat peptide nanoparticles, which were spherical micelles with a size of 50 nm. The degree of hydrolysis of oat protein was 3.1% by pepsin digestion and 10.7% by trypsin digestion. Internal interaction analysis showed that the self-assembly of oat peptide nanoparticles was mainly driven by hydrophobic interaction, and hydrogen bond and disulfide bond played a certain role in maintaining structural stability. The study of surface activity showed that the surface of oat peptide had certain hydrophobicity and lipophilicity, and hydrolysis modification greatly enhanced the water solubility of nanoparticles. Caffeic acid phenethyl ester was used as a model drug to evaluate the drug loading performance of oat peptide nanoparticles. The results showed that entrapment efficiency was 71%, the drug loading was 3.5%, and the concentration of caffeic acid phenethyl ester in water was increased from 1.8 μg/mL to 140 μg/mL. The results of simulated digestion of drug loaded nanoparticles in vitro showed that digestive resistant peptide nanoparticles had strong digestive resistance. Steady state fluorescence spectra showed that hydrophobic interaction was the main binding force between caffeic acid phenethyl ester and peptide molecules. Oat peptide nanoparticles are good carriers to increase the solubility of hydrophobic substances.
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