Effect of co-culture of Clostridium strains and Novisyntrophococcus fermenticellae, isolated from pit mud, on growth and short-chain fatty acid metabolism
SUN Hong1,2, CHAI Lijuan2,4,*, FANG Guanyu1,2, LU Zhenming2,4, ZHANG Xiaojuan2,4, WANG Songtao5, SHEN Caihong5, SHI Jinsong3, XU Zhenghong1,2,5,*
1(School of Biotechnology, Jiangnan University, Wuxi 214122, China) 2(National Engineering Research Center for Cereal Fermentation and Food Biomanufacturing, Jiangnan University, Wuxi 214122, China) 3(School of Life Science and Health Engineering, Jiangnan University, Wuxi 214122, China) 4(Jiangsu Provincial Engineering Research Center for Bioactive Product Processing, Jiangnan University, Wuxi 214122, China) 5(National Engineering Research Center of Solid-State Brewing, Luzhou 646000, China)
Abstract: To study the interaction between Clostridium, the important functional microorganisms in pit mud of Chinese strong-flavor Baijiu, and other microorganisms in pit mud, we analyzed the differences of genomic functions and short chain fatty acid (SCFA) metabolic pathways of Clostridium species based on comparative genomics.Besides, we selected Novisyntrophococcus fermenticellae JN500902 (N.902) and seven strains of Clostridium isolated from pit mud as the research object, constructed a two-strain coculture system of N.902 and Clostridium strain, and compared the changes of growth and SCFA metabolism in mono- and co-culture.Comparative genomics analysis showed that Clostridium’s SCFA metabolic pathway was interspecific differences.Co-culture experiment results further showed that N.902 had a significant positive effect on the growth and metabolism of Clostridium fermenticellae JN500901 (C.901) and C.luticellarii Clu07, the consumption of glucose and growth increased in coculture.Besides, the accumulation of butyric acid and caproic acid in C.901 increased by about 0.44 folds and 0.63 folds, respectively, and the yield of butyric acid and valeric acid in Clu07 increased by 0.77 times and 2.63 times, respectively.N.902 co-cultured with two valeric acid producing Clostridium strains had no significant effect on the growth, while it promoted the production of valeric acid in C.scatologenes Cls01 and inhibited the accumulation of valeric acid in C.aciditolerans Claci01.Co-culture of N.902 and three butyric acid-producing Clostridium strains, C.beijerinckii Clb01, C.guangxiense Clgx01 and C.tyrobutyricum Clt01 showed higher growth rate and biomass than that of mono-culture in the early stage (before 24 h), but the accumulation of butyric acid decreased in varying degrees at the end of co-culture fermentation.The research on the interaction between Clostridium and N.902 could broaden our understanding of pit mud microbial interaction for aroma production.
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SUN Hong,CHAI Lijuan,FANG Guanyu,et al. Effect of co-culture of Clostridium strains and Novisyntrophococcus fermenticellae, isolated from pit mud, on growth and short-chain fatty acid metabolism[J]. Food and Fermentation Industries, 2022, 48(15): 24-32.
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