Abstract: In order to improve the solubility of alcohol leached soybean protein concentrate(ALSPC) and peanut meal(PM),Alcalase and Validase FP concentrate protease were used to limited hydrolyze ALSPC and PM.Results showed that for the Alcalase hydrolyze ALSPC,the optimum temperature,pH value,hydrolysis time,substrate concentration and ratio of Alcalase to substrate were 50℃,8.0,3 h,10%,0.5%,respectively.Under the said conditions,the nitrogen solubility index(NSI) of the obtained protein hydrolysate was improved from 4.37% to 60.34%,and the degree of hydrolysis(DH) is 6.37%.The optimum conditions for the temperature,pH value,hydrolysis time,substrate concentration and ratio of Validase FP concentrate protease to substrate that the Validase FP concentrate protease hydrolyze PM were 50℃,7.0,4h,10%,1%,respectively.Under these conditions,the NSI of the obtained protein hydrolysate was improved from 42.40% to 87.54%,and the DH is 12.90%.
郑芳燕,骆练,刘新征,等. 蛋白酶对大豆蛋白和花生蛋白溶解性的影响[J]. 食品与发酵工业, 2010, 36(10): 75-78.
Zheng Fang-yan,Luo Lian,Liu Xin-zheng,et al. Effect of Protease on Solubility of Soy Protein and Peanut Protein[J]. Food and Fermentation Industries, 2010, 36(10): 75-78.