Abstract: A method to determine the content of hesperidin in citrus peel by capillary electrophoresis was established.The conditions for hesperidin determination,such as buffer,buffer concentration,buffer pH value and separation voltage were investigated.The optimum conditions for hesperidin determination were: borax buffer,buffer pH of 8.0,buffer concentration of 150 mmol/L,separation voltage of 12 kV.Under the optimum conditions,detection limitation was 1.25 μg/mL,the average recovery was 99.75% with RSD of 1.98%.The method is simple,rapid,and accurate.It provides a new method for hesperidin detection in its application of functional food and medicine.
2011, Vol. 37 
