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食品与发酵工业  2011, Vol. 37 Issue (04): 7-11    DOI: 10.13995/j.cnki.11-1802/ts.2011.04.018
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溶氧调控策略对Alcaligenes sp. NX-3产威兰胶发酵过程的影响
万萍,李会,徐浩,陈飞,徐虹
Effect of Dissolved Oxygen Control Strategies on Welan Gum Fermentation by Alcaligenes sp. NX-3
Wan Ping, Li Hui, Xu Hao, Chen Fei, Xu Hong
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摘要 主要研究了发酵过程中溶氧调控策略对Alcaligenes sp.NX-3产威兰胶的影响。在7.5 L发酵罐中,首先考察了不同通气量对菌体生长、威兰胶浓度、葡萄糖消耗、威兰胶黏度等的影响。结果表明,通气量为1 vvm时,有利于威兰胶浓度的提高。通过研究不同供氧水平对威兰胶发酵的影响,发现较高的溶氧水平有利于威兰胶的合成,但是不利于威兰胶黏度的提高。因此提出了低供氧-中供氧-高供氧、高供氧-中供氧-低供氧2种不同溶氧调控策略。实验结果表明:采用高供氧-中供氧-低供氧策略,能够使威兰胶浓度达到25.4 g/L,葡萄糖对威兰胶的转化率提高到0.51 g/g,威兰胶黏度达到2.700 Pa.s。
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万萍
李会
徐浩
陈飞
徐虹
关键词:  威兰胶  Alcaligenes sp. NX-3  溶氧调控策略    
Abstract: The dissolved oxygen control in the production of welan gum by Alcaligenes sp.NX-3 was investigated.Firstly,based on the analysis of the effect of various aeration rate on cell growth,welan gum concentration,glucose consumption,dissolved oxygen level,and welan gum viscosity,we confirmed that in a 7.5 L bioreactor 1 vvm aeration rate was beneficial for welan gum production.After that,the effect of different oxygen supply levels on the welan gum fermentation was studied.The results indicated that high oxygen supply level could improve the welan gum production,but it was not helpful for welan gum viscosity.Therefore,the two oxygen supply strategies:low-moderate-high oxygen supply level and high-moderate-low oxygen supply level were proposed.The results showed that the latter was better for the welan gum fermentation.Finally,the maximum concentration of welan gum reached 25.4 g/L with a yield of 0.51 g/g and the welan gum viscosity of 2.700 Pa·s.
Key words:  welan gum    Alcaligenes sp.NX-3    dissolved oxygen control strategy
               出版日期:  2011-04-25      发布日期:  2011-04-25      期的出版日期:  2011-04-25
引用本文:    
万萍,李会,徐浩,等. 溶氧调控策略对Alcaligenes sp. NX-3产威兰胶发酵过程的影响[J]. 食品与发酵工业, 2011, 37(04): 7-11.
Wan Ping,Li Hui,Xu Hao,et al. Effect of Dissolved Oxygen Control Strategies on Welan Gum Fermentation by Alcaligenes sp. NX-3[J]. Food and Fermentation Industries, 2011, 37(04): 7-11.
链接本文:  
http://sf1970.cnif.cn/CN/10.13995/j.cnki.11-1802/ts.2011.04.018  或          http://sf1970.cnif.cn/CN/Y2011/V37/I04/7
[1] 李会,李莎,冯小海,汪芙蓉,徐虹. Alcaligenes sp.NX-3产威兰胶的补料分批发酵工艺研究[J]. 食品与发酵工业, 2009, 35(1): 1-.
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