Abstract: A sensitive indirect competitive chemiluminescence enzyme immunoassay(CLEIA) for zearalenone in corn has been developed.The optimized CLEIA gave a limit detection of 0.09 ng/mL and a detection range of 0.104-1.69 ng/mL,with an IC50 of 0.438 1 ng/mL and it was about 3 times more sensitive than that of colorimetric ELISA.It has been validated on cereals samples in terms of precision(intra-and interassay coefficient variations of less than 9.1﹪ and 14.7﹪,respectively) and accuracy(mean recovery 79.6﹪~97.6﹪).These assay performances indicated that CLEIA was capable of being applied for the specific detection and routine monitoring of ZEN in corn samples.
卢江,许杨,楚金申,等. 化学发光酶免疫法检测玉米中玉米赤霉烯酮[J]. 食品与发酵工业, 2011, 37(05): 160-165.
Lu Jiang,Xu Yang,Chu Jin-shen,et al. Chemiluminescence Enzyme Immunoassay for the Determination of Zearalenone in Corn[J]. Food and Fermentation Industries, 2011, 37(05): 160-165.