Abstract: Using ultrasonic extraction,O-nornuciferin,N-nornuciferin,nuciferine and roemerine in Nelumbo nucifera leaves were extracted and determined by the established HPLC method.This work provided a new method for quality control of Nelumbo nucifera leaves and its products.Four alkaloids were separated on a reversed-phase Welch Materials C18 column with a gradient elution using acetonitrile-1% triethylamine solution as mobile phase.Linear regression of O-nornuciferin,N-nornuciferin,nuciferine and roemerin in the concentration range of 0.1~50 μg/mL(R>0.999) was satisfied and the detection limit were 1.87 ng,2.22ng,2.34ng and1.88 ng(S/N=3∶ 1),respectively.The method was very precious with good recovery.It can be used for determination of alkaloids from Nelumbo nucifera leaves.
郑振佳,席兴军,赵先恩,等. 超声辅助提取-高效液相色谱法测定荷叶中的生物碱[J]. 食品与发酵工业, 2011, 37(06): 168-171.
Zhang Zhen-jia,Xi Xing-jun,Zhao Xian-en,et al. A Combination of Ultrasonic Assisted Extraction with HPLC in the Determination of Alkaloids from Nelumbo nucifera Leaves[J]. Food and Fermentation Industries, 2011, 37(06): 168-171.