Abstract: Extracted collagen from integument of Asterias amurensis.Prepared Asterias amurensis Collagen Peptides(AACPs) using different enzymes,and investigated antioxidative activity of the AACPs both in vivo and in vitro.Method: The collagen was hydrolyzed by six different kind of enzymes in their optimal conditions.The best enzyme was determined according to AACPs' ability of free radical scavenging and anti-lipid peroxidation effect on rabbit liver.Orthogonal experiment was used to determine the optimum hydrolyzed condition.An acute ethanol-induced oxidative stress mice model was established and was used to evaluated the antioxidant ability of AACPs with the sample that has the highest activity(7d,ig).The activity of SOD,GSH-PX and content of MDA in liver were tested in this study.Result: AACP hydrolyzed with papain has the highest anti-oxidative activity in vitro.Compared with ethanol group,medium-dose and high-dose AACPs significantly reduced the content of MDA(P < 0.01) and increase the activity of SOD(P < 0.05) and GSH-PX(P < 0.01) in liver of mice.Conclusion: Papain was chosen as the best enzyme for producing Asterias amurensis collagen peptides and the obtained AACPs have obvious anti-oxidative activity both in vitro and vivo.
2011, Vol. 37 
