Abstract: The core technology was that the F0F1-ATPase molecular motor biosensor on chromatophore was used to rapidly detect Vbrio cholerae.Specific ompW probe were connected with ε subunit of F0F1-ATPase by avidin-biotin system,and then biosensors were constructed,and the test samples and negative sample were respectively combined with biosensors.After the ATP synthesis was catalyzed by them for 30 min,the content of ATP and Vbrio cholerae DNA in the samples were measured.The amount of ATP synthesis could be measured via the measurement of the amount of H+,while the amount of H+ was normalized by the fluorescence intensity of F-DHPE.According to the results of our experiments,optimum conditions for detection was under the presence of 0.078 mg/mL chro ompW and 40 ng/mL Vbrio cholerae DNA.After detection of the actual samples,we found that the method was in good agreement with the traditional detection methods and PCR detection method.
2012, Vol. 38 
