Abstract: A high-performance liquid chromatographic(HPLC) procedure for determining AY was described.The method was based on a reversed-phase column(Agilent Hypersil ODS column,125 mm×4.6 mm×5 μm) with precolumn derivatization withο-phthaldialdehyde and fluorometric detection(excitation wave length was 340 nm and emission wave length was 450 nm).AY were separated using gradient solution with the flow rate 1.0 mL /min.It allowed simultaneous determination of AY,free amino acids and other peptides in 30 min.Linearity for each amine was observed from 0.1 to 1 000.0 mg/L.Detection limit was 0.005 mg/L.The average recovery of AY from corn oligopeptides was 97.8%.The method was simple,fast,and reliable.It could be used to detect AY in corn oligopeptides.
潘红阳,王树英,江波,等. OPA柱前自动衍生HPLC-FLD法测定玉米低聚肽中AY的含量[J]. 食品与发酵工业, 2012, 38(06): 182-185.
Pan Hong-yang,Wang Shu-ying,Jiang Bo,et al. Determination of AY in Corn Oligopeptides by High-performance Liquid Chromatography-Fluorescence Detector with OPA Precolumn Derivatization[J]. Food and Fermentation Industries, 2012, 38(06): 182-185.