Abstract: Extract sterol in Ganoderma by shear emulsifier was studied. Ergosterol and ergosta-7,22-dien-3β-ol were extracted by GC-MS. On the basis of single factor experiments,response surface methodology was employed to optimize the extraction conditions. The ergosterol yield was 0. 721‰ under the optimal condition( revolving speed of8400r / min,liquid-to-material ratio of 25mL / g,time of 9min). The antioxidative activity of total sterol was compared with VC. When the concentration of total sterol reached to 1 000 μg / mL,the scavenging to DPPH · reached95. 16%,the scavenging to ABTS+· was 81. 33%,the scanvenging to ·OH reached 55. 41%,the reducing power absorbance was 1. 746.
何荣军,周菲,赵月钧,等. 灵芝总甾醇的闪式提取工艺优化及其抗氧化活性[J]. 食品与发酵工业, 2013, 39(09): 200-204.
HE Rong-jun,ZHOU Fei,ZHAO Yue-jun,et al. Study on sterols extraction with high-speed shearing and antioxidation in ganoderma[J]. Food and Fermentation Industries, 2013, 39(09): 200-204.