Abstract: In this study,the pit mud of Luzhou-flavour liquor which had been used for 1,50,100 and 400 years were taken from a factory affiliated to the Luzhou Laojiao Group. Eight mixed samples were sampled from the wall and bottom layers of the pit mud in the cellars. PCR-DGGE was employed to analyse Archaea community structure and diversity in the samples. DGGE analysis showed that both of the diversity index( H) and the evenness index( EH) of the bottom-layer sample was higher than those of the wall-layer samples from the same cellar. However,in different cellar,the discrepancy of H values between samples taken from the same position was significant,and the variation of H values of the wall and the bottom layers were between 2. 54 to 2. 86 and 2. 76 to 3. 23,respectively. In addition,with the increase of pit age,the EH values gradually increased from 0. 985 to 0. 991 in the wall-layer sample,and from 0. 991 to 0. 995 in the bottom-layer sample. Moreover,those samples taken from the same cellar exhibited a higher level of the similarity coefficient( S C),ranging form 0. 36 to 0. 74,and the S C values of samples from the wall and the bottom layers were between 0. 42 to 0. 63 and 0. 36 to 0. 52,respectively.
刘茂柯,唐玉明,邹兰,等. 浓香型白酒窖泥古菌群落结构及其多样性[J]. 食品与发酵工业, 2013, 39(10): 22-28.
LIU Mao-ke,TANG Yu-ming,ZOU Lan,et al. Archaea community and diversity in the pit mud of Chinese Luzhou-flavour liquor[J]. Food and Fermentation Industries, 2013, 39(10): 22-28.