Abstract: Collagen was prepared from the pigskin using enzyme method,and its purity was detected by SDSPAGE. Then,differential scanning calorimetry( DSC) was used to study the influence of holding temperature(- 0.6,- 0. 5,- 0. 4,- 0. 3,- 0. 2℃),collagen concentrations( 0. 1,0. 5,1,2. 5,5,10,15,20,50 mg / mL) and rate( 0. 5,1,2. 5,5,10℃ / min) on the thermal hysteresis activity( THA). The results suggested that the prepared pigskin collagen was a typical type I collagen and the molecular weight of collagen was about 330 KD with higher purity. A maximum THA of 0. 61℃ can be obtained with the protein concentration of 20 mg / ml,holding temperature of0. 12℃ and the heating and cooling rate of 10℃ / min. The choice of holding temperature was critical for effective measurement of THA,while the heating and cooling rates had almost no impact on the THA.