Abstract: Twenty-five phospholipase-producing strains were isolated from the soil samples collected in different regions by the method of egg yolk plate. One strain JYSY1 was selected for the high growth and production of phospholipases. The strain JYSY1 was preliminarily identified as Bacillus cereus based on the morphological characteristics,physiological and biochemical analysis,and sequence analysis of 16S rDNA. The optimum temperature for cell growth and phospholipase production were 25 ℃ and 30 ℃. The optimum pH value for the cell growth and phospholipase production was pH 7. 0. A phospholipase was purified from the culture supernatant of the strain JYSY1 by means of ammonium sulfate precipitation,column chromatography on DEAE-Sepharose Fast Flow and SephadexG100. The enzyme appeared to be a single peptide of 48 kDa. The optimal temperature and pH for the enzyme reactivity were 50 ℃ and 8. 0,respectively. Addition of Co2 +and Ca2 +increased its activity to 111. 59% and 110. 11%respectively,but Zn2 +and Mg2 +respectively inhibited the enzymatic activity. Addition of 1mmol /L of PMSF,SDS,EGTA and EDTA also inhibited the enzymatic activity. At 65 ℃,about 55% enzyme activity was remained. The purified enzyme was identified to be a phospholipase C. The enzyme was suggested to be a metallophospholipase.
杨艳艳,陈吉祥,杨智,等. 热稳定性磷脂酶生产菌的分离鉴定及酶学性质研究[J]. 食品与发酵工业, 2014, 40(05): 48-54.
YANG Yan-yan,CHEN Ji-xiang,YANG Zhi,et al. Studies on screening of a thermostable phospholipase producing bacterium and its enzyme characterizatics[J]. Food and Fermentation Industries, 2014, 40(05): 48-54.