Abstract: β-galactosidase can be employed to produce galacto-oligosaccharidess using lactose as the substrate.Previously,a recombinant P. pastoris strain containing the gene of β-galactosidase mutant F441 Y from Sulfolobus solfataricus was constructed in our lab. In this study,whole cells of recombinant P. pastoris were used as biocatalyst to produce galacto-oligosaccharides,and the conditions such as initial p H,temperature,lactose concentration,enzyme concentration and reaction time were optimized. The optimal conditions were as follows: 80%( w / v) lactose,p H6. 0,80℃,5U / m L enzyme. After reaction for 24 h,the yield of GOS reached 59. 9%. Compared with the GOS production process using free enzyme,the proposed transformation approach with whole cells exhibited less impurity proteins and Maillard reaction,which made it easier for the separation of GOS. This study laid a foundation for the application of β-galactosidase.
段绪果,孙晓军,吴敬. β-半乳糖苷酶全细胞催化制备低聚半乳糖工艺优化[J]. 食品与发酵工业, 2014, 40(12): 61-65.
DUAN Xu-guo,SUN Xiao-jun,WU Jing. Optimization of production of galacto-oligosaccharides using whole cells of a recombinant Pichia pastoris as catalyst[J]. Food and Fermentation Industries, 2014, 40(12): 61-65.
2014, Vol. 40 
