Abstract: The production of L-erythrulose by resting cells of Gluconobacter kondonii was optimized using response surface design. On the basis of single factor test,the Response Surface Methodology( RSM) was designed for the first time,and the mathematical model was set up between various factors and L-erythrulose. The results indicated that the effects of the concentration of resting cells and erythritol,p H value,reaction time and temperature on the production of L-erythrulose were significant. Response Surface prediction showed that the optimal conditions for production of Lerythrulose were as follows: the concentration of resting cells 1. 65%,the concentration of erythritol 97. 9 g / L,temperature 30. 2℃,p H value 5 and the reaction time 18 h. Under these conditions,the yield of L-erythrulose reached96. 1 g / L,and the response surface extremes coincide conversion was 98. 2%,production intensity was 5. 34 g /( L·h). All of these showed that optimization program achieved the desired effect.
潘龙,靳魁奇,刘宇鹏. 静息细胞转化赤藓糖醇生产L-赤藓酮糖条件优化[J]. 食品与发酵工业, 2014, 40(12): 72-76.
PAN Long,JIN Kui-qi,LIU Yu-peng. Research on bioconversion conditions for the production of L-erythrulose from erythritol with resting cells[J]. Food and Fermentation Industries, 2014, 40(12): 72-76.
2014, Vol. 40 
