Abstract: A high performance liquid chromatography( HPLC) method has been developed for the detection of five eugenolanesthetic residuessimultaneous: Eugenol,Iso-eugenol,Methyl eugenol,Methyl iso-eugenol,Acetyl iso-eugenol in aquatic products. Sample was ultrasonic extracted by acetonitrile,the fat was cleaned up twice by n-hexane,and then the diluted solution was determined by HPLC. External standard calibration was used in the determination.Five kinds of eugenol type of anesthetic showed good linearity in the concentrations range of 0. 05 ~ 10. 00 μg / m L in the standard solutions carves,and all of the correlation coefficents( R2) were higher than 0. 999 0. At the spiked levels of 0. 10 ~ 1. 00 mg / kg,the average recoveries were 80. 76% ~ 102. 63%. The relative standard deviations were0. 25% ~ 5. 49%. The limits of quantificationg( LOQ) of Eugenol,Iso-eugenol and Methyl iso-eugeno were 0. 05 mg /kg,and Methyl eugenol and Acetyl iso-eugenol were 0. 10 mg / kg. The method is suitable for the detection of batch clove phenol drug residues in aquatic products.