Abstract: The aim of this study was to establish an accurate and effective method for determining Vitamin K1 in dairy products. After extraction,the samples were analyzed by C18,with methanol as mobile phase. The absorption wavelength of 248 nm was chosen to detect vitamin K1. The results showed that calibration curves were linear between0 ~ 4. 0μg / m L,and the correlation coefficients were 0. 9996. The recoveries of the vitamin K1 in the dairy products were more than 90. 0% with relative standard deviations less than 2. 0%. This method is better than the national standard method GB 5413. 10-2010 in terms of precision and recovery. In addition,it can simultaneously determine the level of vitamin A,vitamin D and vitamin E. This is an effective and accurate method for determination of vitamin K1.
吴海智,冉丹,袁列江,等. 高效液相色谱法测定乳制品中V_(K_1)方法的改进[J]. 食品与发酵工业, 2014, 40(12): 161-164.
WU Hai-zhi,RAN Dan,YUAN Lie-jiang,et al. Research on analysis improvement of vitamin K_1 in dairy products by HPLC[J]. Food and Fermentation Industries, 2014, 40(12): 161-164.