Please wait a minute...
 
 
食品与发酵工业  2014, Vol. 40 Issue (12): 169-176    DOI: 10.13995/j.cnki.11-1802/ts.201412033
  食品与发酵工业 本期目录 | 过刊浏览 | 高级检索 |
江苏沿海4种低值贝类软体中不饱和脂肪酸EPA和DHA动态积累规律与适宜采收期分析
李娜,王欣之,吴皓,易瑞灶,程建明,刘睿,王令充,谢冬云,陈涛
Evaluation of the dynamic accumulation of polyunsaturated fatty acid EPA and DHA in four shellfish in the coast of Jiangsu as well as their optimal harvest times
LI Na, WANG Xin-zhi, WU Hao, YI Rui-zao, CHENG Jian-ming, LIU Rui, WANG Ling-chong, XIE Dong-yun, CHEN Tao
下载:  HTML   PDF (496KB) 
输出:  BibTeX | EndNote (RIS)      
摘要 采用GC/MS法对江苏沿海四种低值贝类软体中脂肪酸类成分进行定性分析,建立了HPLC法测定4种贝类软体中多烯不饱和脂肪酸二十碳五烯酸(简称EPA)和二十二碳六烯酸(简称DHA)的方法,在此基础上分析不同采收期4种贝类软体EPA和DHA的动态积累规律以评价这4种贝类软体的适宜采收期;同时以Waters Sun Fire C18柱(2.1 mm×100 mm,3.5μm)为色谱柱,乙腈(含0.1%磷酸)-水(含0.1%磷酸)(体积比74∶26)为流动相,流速0.6 m L/min等度洗脱,检测波长205 nm。该方法下,EPA-甲酯和DHA-甲酯分别在1.005 0~80.40μg/m L、1.106 2~88.50μg/m L范围内呈良好的线性关系,灵敏度高,重现性好。不同的采收期,4种贝类软体中EPA和DHA的含量变化明显,在6.7月份时达到最高,菲律宾蛤仔、文蛤、青蛤和四角蛤蜊软体中EPA和DHA的总量分别为:771.56~4 565.38μg/g、263.11~3 115.78μg/g、5 907.83~12 023.06μg/g和422.71~2 416.27μg/g。从产量和有效成分EPA和DHA的总量变化上综合考虑,建议菲律宾蛤仔、青蛤和四角蛤蜊最佳采收期在6月,而文蛤最佳采收期在7月。
服务
把本文推荐给朋友
加入引用管理器
E-mail Alert
RSS
作者相关文章
李娜
王欣之
吴皓
易瑞灶
程建明
刘睿
王令充
谢冬云
陈涛
关键词:  HPLC  二十碳五烯酸  二十二碳六烯酸  贝类软体  采收期    
Abstract: The qualitative analysis of free fatty acids in four shellfish flesh in the coast of Jiangsu was performed using GC / MS method. Moreover,the HPLC method to determine cis-5,8,11,14,17-eicosapentaenoic acid( EPA)and cis-4,7,10,13,16,19-docosahexaenoic acid( DHA) in these shellfish flesh was first established; the dynamic accumulation law of polyunsaturated fatty acid EPA and DHA in four shellfish flesh was tested to find out the optimal harvest time. The Waters Sun Fire C18column( 2. 1 mm × 100 mm,3. 5 μm) was used at a flow rate of 0. 6 m L / min with acetonitrile( containing 0. 1% phosphoric acid)-water( containing 0. 1% phosphoric acid)( 76 ∶ 24) as the mobile phase. The eluent was detected at 205 nm. By the above-mentioned method,EPA-methyl ester and DHAmethyl ester exhibited good linear relationship within the range of 1. 005 0 ~ 80. 40 μg / m L and the range of 1. 106 2~ 88. 48 μg / m L respectively,with high sensitivity and good reproducibility. The total contents of Eicosapentaenoic acid and Docosahexaenoic acid in four shellfish flesh were significantly different in different harvest time,where June or July turned out to be the optimal harvest time. The total contents of EPA and DHA in Ruditapesphilippinarum ranged from 771. 56 μg / g to 4 565. 38 μg / g and,Meretrixmeretrix linnaeus,Cyclinasinensis and Mactraveneriformis reeve was 263. 11 μg / g to 3 115. 78 μg / g,5 907. 83 μg / g to 12 023. 06 μg / g and 422. 71 μg / g to 2 416. 27 μg / g,respectively. Based on the production and total contents of effective constituents( EPA and DHA),the best harvest time for Ruditapesphilippinarum,Cyclinasinensis,Mactraveneriformis reeveisin June,and Meretrixmeretrix linnaeusis in July.
Key words:  HPLC    eicosapentaenoic acid    docosahexaenoic acid    shellfish flesh    harvest time
               出版日期:  2014-12-25      发布日期:  2014-12-25      期的出版日期:  2014-12-25
引用本文:    
李娜,王欣之,吴皓,等. 江苏沿海4种低值贝类软体中不饱和脂肪酸EPA和DHA动态积累规律与适宜采收期分析[J]. 食品与发酵工业, 2014, 40(12): 169-176.
LI Na,WANG Xin-zhi,WU Hao,et al. Evaluation of the dynamic accumulation of polyunsaturated fatty acid EPA and DHA in four shellfish in the coast of Jiangsu as well as their optimal harvest times[J]. Food and Fermentation Industries, 2014, 40(12): 169-176.
链接本文:  
http://sf1970.cnif.cn/CN/10.13995/j.cnki.11-1802/ts.201412033  或          http://sf1970.cnif.cn/CN/Y2014/V40/I12/169
[1] 熊善波, 肖全伟, 何鲒, 汪佳林, 何瀚庭, 兰航. 微波萃取结合高效液相色谱-电感耦合等离子体质谱法分析测定干制食用菌中汞的形态[J]. 食品与发酵工业, 2020, 46(22): 252-256.
[2] 石兵艳, 李祥, 刘毅, 王芸. 八月瓜果皮中常春藤皂苷元水解工艺优化及图谱分析[J]. 食品与发酵工业, 2020, 46(2): 239-245.
[3] 郑晓杰, 林胜利, 吴聪聪, 聂小华. 青钱柳叶抗氧化物质积累的差异性研究[J]. 食品与发酵工业, 2020, 46(11): 238-243.
[4] 葛敏敏, 王建华, 林洪. 液相色谱串联质谱检测牙鲆鱼中的小清蛋白过敏原[J]. 食品与发酵工业, 2019, 45(8): 210-214.
[5] 牛丽影, 刘春菊, 李大婧, 宋江峰, 刘春泉. 不同采收期鲜食玉米氨基酸变化分析[J]. 食品与发酵工业, 2019, 45(6): 209-214.
[6] 吴佳佳, 王思齐, 戴志远. 咸鳓鱼中产组胺菌的分离与鉴定[J]. 食品与发酵工业, 2019, 45(3): 71-76.
[7] 郑连姬, 邹勇, 张琪, 李智, 费华熙, 钟耕. 氧化铝柱-高压液相色谱法测定重庆火锅底料中7种非食用色素及暴露评估[J]. 食品与发酵工业, 2019, 45(19): 277-286.
[8] 任艳娇, 刘玉淑, 伍姚, 喻卉, 李伟丽, 吴韬. 超高效液相色谱-串联四级杆飞行时间质谱测定奶粉中N-乙酰神经氨酸[J]. 食品与发酵工业, 2019, 45(19): 287-291.
[9] . 柑橘果肉、果皮中酚类物质含量差异性研究[J]. 食品与发酵工业, 2018, 44(4): 241-247.
[10] 朱丹凤, 王园园, 崔树茂, 毛丙永, 赵建新, 张灏, 陈卫. 罗伊氏乳杆菌氮源利用的选择性与特征分析[J]. 食品与发酵工业, 2018, 44(11): 35-41.
[11] 胡学超, 任路静, 胡耀池, 纪晓俊, 黄和. 裂殖壶菌制备二十二碳六烯酸油脂的研究历程及发展前景[J]. 食品与发酵工业, 2018, 44(11): 307-312.
[12] 李辉, 张静, 李超, 等.. 贺兰山东麓不同陈酿年份赤霞珠干红葡萄酒中酚类物质 对涩感质量的影响[J]. 食品与发酵工业, 2018, 44(10): 38-44.
[13] 代娟,田艳萍,张小春,袁亚. 蕨菜中原蕨苷含量的测定及加工过程对原蕨苷含量的影响[J]. 食品与发酵工业, 2017, 43(9): 149-.
[14] 张南海,涂宗财,何娜,王辉,黄涛,李翠. 尿素包合法富集鲢鱼鱼油中多不饱和脂肪酸工艺优化[J]. 食品与发酵工业, 2017, 43(6): 152-.
[15] 王静莹,薄海波,吉生军,程子毓,陈秀红. 高效液相色谱法测定牛羊杂碎等肉类中嘌呤及尿酸[J]. 食品与发酵工业, 2017, 43(4): 232-.
No Suggested Reading articles found!
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
版权所有 © 《食品与发酵工业》编辑部
地址:北京朝阳区酒仙桥中路24号院6号楼111室
本系统由北京玛格泰克科技发展有限公司设计开发  技术支持:support@magtech.com.cn