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N+注入诱变选育氨肽酶高产菌株及发酵条件初步优化 |
吴庆勋,谷海先,赵光鳌 |
江南大学工业生物技术教育部重点实验室,无锡,214036 |
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Breeding of Aminopeptidase-producing Strains by N + Implantation and Preliminary Research on Fermentation Conditions |
Wu Qingxun,Gu Haixian,Zhao Guangao |
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摘要 通过N+注入米曲霉WJ05-1,筛选得到2株氨肽酶活力提高了30%的高产菌株M60-5-13和M80-10-7,经多次传代实验表明2菌株遗传稳定性良好.对M80-10-7的发酵条件,如碳氮源及浓度、起始pH、发酵时间和表面活性剂等进行了初步优化,进一步使产酶水平提高了77.5%.
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出版日期: 2006-01-25
发布日期: 2017-12-01
期的出版日期: 2006-01-25
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