|
|
|
| 酵母生物转化合成2-苯乙醇的培养条件优化 |
| 崔志峰,沈情佳,杨霄,车智博,汪琨 |
| 浙江工业大学生物与环境工程学院,浙江,杭州,310032 |
|
| Optimization of the Medium and Conditions for the Production of 2-phenylethanol by Yeast |
| Cui Zhifeng,Shen Qingjia,Yang Xiao,Che Zhibo,Wang Kun |
|
|
|
摘要 通过单因素试验和均匀设计试验对酿酒酵母Saccharomyces cerevisiae CWY132生物转化合成2-苯乙醇的培养基组成及培养条件进行优化研究.优化后培养基组成及培养条件为:葡萄糖30.1 g/L,KH2PO4 5 g/L,L-苯丙氨酸5.8 g/L,MgSO4 0.5 g/L,酵母氟碱0.17 g/L;最佳初始pH 5～6,接种密度1.21×107/mL,最适培养温度28～30℃,200 r/min振荡培养36 h.优化后2-苯乙醇产量达到3.98 g/L,比优化前的1.9 g/L提高了109%.原料L-苯丙氨酸的摩尔转化率从最初的51.4%提高到了92.7%.
|
|
|
出版日期: 2008-08-25
发布日期: 2017-12-01
期的出版日期: 2008-08-25
|
| No Suggested Reading articles found! |
|
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
| |
Shared |
|
|
|
|
| |
Discussed |
|
|
|
|
2008, Vol. 34 
