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| 高效液相色谱-蒸发光散射检测法测定玉米浆中游离L-精氨酸 |
| 万红贵,宗素艳,朱明新,袁建锋,蔡恒 |
| 南京工业大学制药与生命科学学院,江苏南京,210009 |
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| Determination of L-arginine in Corn Steep Liquor by High Performance Liquid Chromatography-evaporative Light-scattering Detector |
| Wan Honggui,Zong Suyan,Cai Heng,Zhu Mingxing,Yuan Jianfeng |
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摘要 建立了高效液相色谱一蒸发光散射(HPLC-ELSD)测定玉米浆中游离L一精氨酸(L-Arg)的检测方法.采用Alltima C18(250 mm×4.6 mm i.d.,5μm)色谱柱,以5.0 mmol/L七氟丁酸的体积分数0.2%三氟乙酸溶液和乙腈(体积比为93.75,6.25)为流动相,流速为0.8 mL/min,ELSD漂移管的温度为110℃,载气流速为2.8 L/min.在该色谱条件下,L-精氨酸浓度50.0～500.mg/L时,其浓度的自然对数与峰面积的自然对数线性关系良好(R2=0.999 6),回收率为97.70%～102.2%,精密度RSD＜2.48%.
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出版日期: 2009-02-25
发布日期: 2017-12-01
期的出版日期: 2009-02-25
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2009, Vol. 35 
