Abstract: Through leaf surface disinfection,strain isolation and purification,ITS rDNA and 26S rDNA D1 /D2region sequence determination and phylogenetic analysis,a priliminary study on the diversity of culturable entophytic fungus community in seeds of Xisha wild Noni was conducted. 48 entophytic fungus strains were isolated,among which39 strains were yeasts,9 strains were molds. 39 yeast strains were belong to 5 species in 4 genera. The dominant yeasts( abundance) were Eremothecium coryli( 31. 25%),Pseudozyma aphidis( 27. 08%) and Cryptococcus flavescens( 16. 67%). 9 mold strains belonged to 4 species in 4 genera. The dominant molds( abundance) were Phaeoacremonium sp.( 8. 33%) and Gibberella sp.( 6. 25%). The results indicated that there was a significant diversity in culturable entophytic fungus community in seeds of Xisha wild Noni and the dominant entophytic fungus were yeasts.Parts of those strains had potential pharmaceutical value.