Abstract: In this study,the alkaloids were extracted from Lentinula edodes via accelerated solvent extraction( ASE) and the response surface methodology( RSM) was used to optimize extraction conditions. The antioxidant activities were measured by DPPH,ABTS and superoxide anion radical scavenging capacity. ASE method can shorten the extraction time and improve extraction rate of total alkaloids. The results showed that the optimum conditions were: ethanol concentration 80%,extraction temperature at 140℃,static extraction time 20 min,extraction twice,blow volume fraction 80%. Under the above conditions,the alkaloids showed significant antioxidant activities.
何晋浙,冯婷婷,孙培龙. 香菇总生物碱提取工艺优化及其抗氧化研究[J]. 食品与发酵工业, 2014, 40(05): 229-234.
HE Jin-zhe,FENG Ting-ting,SUN Pei-long. Extracting technology and antioxidant activity of total alkaloids from Lentinula edodes[J]. Food and Fermentation Industries, 2014, 40(05): 229-234.