To analyze the mechanism of the microbial community stability during the formation of broad bean paste,filter paper method and oxford cup method were used.Touch-down PCR (TD PCR) was also performed to detect the genes encoding certain antibiotics including subtilosin,subtilin,surfactin and plipastatin.Results showed that all the anaerobic bacteria and yeast could be inhibited by one or more aerobic bacteria,such as Bacillus subtilis HYM05,B.amyloliquefaciens HYM24,and B.tequilensis HYM42.TD PCR detection indicated that the genes encoding subtilosin and plipastatin existed in the genome of HYM42 and HYM05.
FAN Zi-hao,ZHU Lin-jiang,LIU Si-yuan,WANG Jia-qi,LI Qi.
Interaction analysis of the strains isolated from traditional fermented broad bean Paste[J]. Food and Fermentation Industries, 2017, 43(4): 28