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食品与发酵工业  2017, Vol. 43 Issue (6): 1-    
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连续离子交换法分离发酵液中的L-精氨酸
黄根树,徐美娟,杨套伟,张显,饶志明,许正宏
江南大学,工业生物技术教育部重点实验室,江苏无锡,214122
江南大学生物工程学院,江苏无锡,214122
Recovery of L-arginine in the fermentation broth by continuous ion exchange
HUANG Gen-shu,XU Mei-juan,YANG Tao-wei,ZHANG Xian,RAO Zhi-ming,XU Zheng-hong
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摘要 相比传统的固定床离子交换提取工艺,连续离子交换工艺分离发酵液中L-精氨酸具有连续、稳定、高效等优点.钝齿棒杆菌是1株具有自有知识产权的高产L-精氨酸工业用菌株,产量达74.5 g/L.该研究通过静态吸附及解吸实验,筛选得到最优树脂D155,可在30 min内达到吸附平衡,平衡吸附量达121.31 mg/g.通过固定床实验,确定了最佳进料流速为6 mL/min,穿透时间为29 min,半饱和时间为59 min,饱和时间86 min,穿透吸附容量为134.7 mg/g,半饱和吸附容量为193.8 mg/g,饱和吸附容量为261.2 mg/g,为最佳洗脱剂为2 mol/L的氨水,洗脱率为97.14%.通过30柱连续离子交换实验,确定了交换区、交换后水洗区、洗脱区、洗脱后水洗区、顶水区最佳进料流速分别为6、6、5、10、3 mL/min.在各区最佳进料流速下,各出口浓度呈周期性变化.该工艺提高了L-精氨酸分离工艺的连续性、稳定性及分离效率,有效地降低了分离过程中的耗水量、耗碱量及劳动力投入,为发酵生产L-精氨酸提供了更科学有效的分离工艺.
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Abstract: Compared with the conventional fixed bed ion exchange process,continuous ion exchange process is better in continuity,stability and efficiency.Corynebacterium crenatum,a excellent L-arginine producer with domestic autonomous intellectual property right,is able to produce L-arginine with a production of 74.5 g/L.In this study,D155 was proved to be the most competent resin to recover the L-arginine in fermentation by static adsorption and desorption tests.Adsorption equilibrium could be achieved within 30 min with the equilibrium capacity of 121.31 mg/g.The optimal feed flow rate obtained from the fixed bed tests was 6 mL/min,at which the breakthrough time,subsaturation time,saturation was 29,59,86 min,respectively.The corresponding breakthrough capacity,subsaturation capacity and saturation capacity was 134.7,193.8,261.2 mg/g,respectively.The optimal eluent was 2 mol/L NH3 ·H2O,which could result in elution rate of 97.14%.Based on the optimal conditions obtained in fixed bed experiments,continuous ion exchange system consisted of 30 columns was designed,and the optimal flow rates of adsorption zone,rinsing zone after adsorption,desorption zone,rinsing zone after desorption,excluding water zone were 6,6,5,10,3 mL/min,respectively.The designed continuous ion exchange system could operate steady at the optimal flow rates,and the outlet concentration of each zone changed periodically.A more scientific recovery process of L-arginine in industrial fermentation was obtained herein,which not only improved the process continuity,stability and efficiency,but also reduced the consumption of water,base and labour.
               出版日期:  2017-06-25      发布日期:  2017-12-01      期的出版日期:  2017-06-25
基金资助: 国家高技术研究发展计划(863计划)(2015AA021004)
国家自然科学基金(31300028)
江苏省自然科学基金(BK20150002,BK20130137)
教育部重点研究项目(113033A)
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