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食品与发酵工业  2017, Vol. 43 Issue (6): 146-    
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富锌培养对香菇菌丝体生长和抗氧化酶系的影响
冮洁,张爽,刘瑶,解彬,庞士磊
大连民族大学,生物技术与资源利用教育部重点实验室,辽宁大连,116600
Effects of zinc on the growth and antioxidant enzymes of Lentinus edodes mycelium
GANG Jie,ZHANG Shuang,LIU Yao,XIE Bin,PANG Shi-lei
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摘要 通过对香菇菌丝体进行液体和固体富锌培养,以确定香菇菌丝体在外源锌的作用下生长和抗氧化酶活性的变化规律.香菇菌丝体具有较强的耐锌和富锌能力,低浓度锌对香菇菌丝体的生长有促进作用,高浓度有抑制作用,适宜的锌质量浓度为600 mg/L.在锌质量浓度400 ~1 000 mg/L,香菇菌丝体超氧化物歧化酶(Superoxide dismutase,SOD)、过氧化物酶(Peroxidase,POD)和过氧化氢酶(Catalase,CAT)随锌浓度增加先升高再降低,最大值分别为:147.47 U/g、6.15×10-3 U/g和147.52 U/g;菌丝体丙二醛(Malondialdehyde,MDA)含量随锌浓度增加先呈下降的趋势,然后逐渐上升,在锌质量浓度600 mg/L时达到最低,为4.47×10-5 μmol/g.香菇菌丝体抗氧化酶系随培养时间变化规律的研究结果表明,香菇菌丝体在培养的第9天,SOD、POD和CAT的酶活性均达最大值,MDA含量达到最低值,分别为:154.31 U/g、6.21×10-3 U/g、147.92 U/g和4.18×10-5μmol/g.香菇菌丝体在适宜浓度富锌培养,不仅对香菇菌丝体的生长有促进作用,同时可提高香菇菌丝体的锌含量,并且提高了香菇菌丝体的抗氧化酶活性.
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Abstract: The objective of this study was to determine the growing changes and the antioxidant enzyme activities of Lentinus edodes mycelium cultured in liquid and solid zinc-rich medium supplemented with exogenous zinc.The results showed that Lentinus edodes mycelium had strong resistance to zinc and zinc-rich ability,while the low zinc concentration could promote the mycelial growth of Lentinula edodes,and the high zinc concentration could inhibit the growth,and the optimum concentration of zinc sulfate was 600 mg/L.The activities of superoxide dismutase (SOD),peroxidase (POD) and catalase (CAT) of Lentinula edodes mycelium increased with the increase of zinc sulfate concentration in the range of 400 mg/L to 1 000 mg/L,and the maximum values were 147.47 U/g,6.15 × 10-3 U/g,147.52 U/g,respectively.The content of malondialdehyde (MDA) decreased with the increase of zinc concentration,and then increased gradually,and the minimum content of MDA was 4.47 × 10-5 μmol/g at a zinc concentration of 600 mg/L.SOD,POD and CAT activities of Lentinula edodes mycelia reached the maximum value and the MDA content reached the lowest value on the 9 th day of culture,which were 154.31 U/g,6.21 × 10-3 U/g,147.92 U/g and 4.18 x 10-5 μmol/g,respectively.The optimal zinc concentration in the medium could improve Lentinus edodes mycelium growth and the zinc content of Lentinus edodes mycelium,and increase the antioxidant enzyme activities.
               出版日期:  2017-06-25      发布日期:  2017-12-01      期的出版日期:  2017-06-25
基金资助: 辽宁省自然科学基金资助项目(2015020676)
财政专项-中央高校基本科研业务费资助项目(DC201501020301)
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