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食品与发酵工业  2017, Vol. 43 Issue (6): 41-    
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海洋脂肪酶YS2071的固定化及酶学性质研究
姜峻颖,马子宾,孙晶晶,郝建华,刘均忠,王跃军,孙谧
中国水产科学研究院黄海水产研究所,海洋国家实验室海洋药物与生物制品功能实验室,山东青岛,266071;上海海洋大学食品学院,上海,201306
中国水产科学研究院黄海水产研究所,海洋国家实验室海洋药物与生物制品功能实验室,山东青岛,266071
The immobilization and enzymatic properties of lipase YS2071
JIANG Jun-ying,MA Zi-bin,SUN Jing-jing,HAO Jian-hua,LIU Jun-zhong,WANG Yue-jun,SUN Mi
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摘要 为提高脂肪酶的利用效率,更好应用于工业化生产,研究了脂肪酶YS2071的固定化技术,筛选最优载体,通过单因素实验法对脂肪酶固定化的条件进行优化,并对其性质进行了研究.采用MI-BSI伯胺功能基吸附树脂为载体,京尼平为交联剂,吸附-交联的方法,分析了加酶量、吸附时间、吸附温度、初始pH、交联剂的浓度、交联时间等因素对脂肪酶固定化效果的影响,并通过PB实验和响应面实验,确定了最佳固定化条件:加酶量为8 mg,吸附时间8h,吸附温度为20℃,初始pH 8.6,交联剂质量浓度为0.48 g/L,交联时间为1h时,固定化效率最高,酶活回收率达到60%以上.
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Abstract: Free lipase is difficult to achieve continuous operation.In order to improve the efficiency of lipase and apply it to the industrialized production,the technology of immobilized lipase YS2071 was studied by screening the optimal carrier and using single factor experiment method to optimize lipase immobilized conditions.Using MI-BSI resin as carrier and genipin as cross-linking agent,adsorption-crosslinking method was employed to maintain relative high enzyme activity.Immobilized conditions were studied by using PB experiment and the response surface design.The optimal conditions of immobilized lipase YS2071 were as followes:the enzyme dosage was 8 mg,the adsorption time was 8 h,the adsorption temperature was 20 ℃,the initial pH was 8.6,the concentration of genipin was 0.48 g/L,the crosslinking time was 1 h.Under these conditions,the recovery rate of immobilized enzyme was up to 60%.
               出版日期:  2017-06-25      发布日期:  2017-12-01      期的出版日期:  2017-06-25
基金资助: NSFC-山东联合资助海洋研究中心项目(U1406402-5)
国际科技合作与交流专项(2014DFG30890)
国家实验室-鳌山科技计划(2015ASKJ02-06)
国家863计划(2014AA093516)
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