To explore the Lactic acid bacteria diversity and biofilm formation in traditional fermented milk from Xinjiang longevity region,the samples were analyzed using pure culture and 16S rRNA gene sequence analysis,96 well micro-plate semi-quantitative methods and scanning electron microscopy.Finally 57 strains were isolated from four samples.The micro-plate semi-quantitative method was used to investigate biofilm formation of strains.The resuits showed that 38 strains (73.68%) of the LAB showed biofilm formation ability,of which 16 strains (28.07%) strongly adhered to biofilm and 22strains (38.59%) were moderately adherent.The strains which can form biofilm were belonging to three species of six genera including Enterococcus durans (16 strains),Enterococcusffaecium (1 strains),Enterococcusthailandicus (7 strains),Enterococcus lactis (3 strains),Lactobacillus plantarum (6 strains),Streptococcus thermophilius (5strains),in which Enterococcus was the dominant species and was followed by Lactobacillus and Streptococcus.These provided foundation for the development and utilization of LAB resources and their biofilm characteristics.
XIRINAY · Ablikim,MUYESSER · Yusup,NURGUL · Rahman.
Screening and identification of lactic acid bacteria forming biofilm in traditional fermented milk in South Xinjiang[J]. Food and Fermentation Industries, 2017, 43(7): 128