Abstract: The recovery of peptides from soy protein hydrolysates (SPHs) under different epigallocatechin-3-gallate (EGCG) concentrations and pH values were studied. The recovery rate of the peptide, the change of the secondary structure content, the surface hydrophobicity and the antioxidant activity were characterized. The results showed that increasing the enzymatic hydrolysis time could increase the intermediate peptide content. And the addition of EGCG also increased the recovery rate of SPHs. In addition, the amount of SPHs was positively correlated with the concentration of EGCG. With the increase of enzymatic hydrolysis time, the recovery rate of the peptide first tend to decrease and then increase, and the recovery rate was the highest at 30 min. It was indicated that EGCG might be easier to precipitate with peptides with a molecular weight of 5-10 kDa. To a certain extent, the addition of EGCG changed the secondary structure of the protein peptide, and the protein peptide was stretched. The addition of EGCG would also reduce the surface hydrophobicity of the SPHs and increase the antioxidant activity. This study provided a reference for EGCG to recover peptides from SPHs by constructing and analyzing the structure and function of SPHs-EGCG and its influence on the recovery rate of peptides.
王佳悦,董亚博,付元涛,等. 利用表没食子儿茶素-3-没食子酸酯回收大豆蛋白酶解液中多肽的研究[J]. 食品与发酵工业, 2021, 47(16): 78-83.
WANG Jiayue,DONG Yabo,FU Yuantao,et al. Study on recovery of peptides from soybean proteolysis solution with epigallocatechin-3-gallate[J]. Food and Fermentation Industries, 2021, 47(16): 78-83.
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