Abstract: Quinoa husks protein was extracted by high concentration ethanol from quinoa husks, and the protein was hydrolyzed by four kinds of proteases (alkaline, neutral, compound and flavor protease) to produce polypeptides. The molecular weight distribution and amino acid composition of quinoa protein, the enzymatic hydrolysis ability of four proteases and the activities of the polypeptides were determined. The results showed that quinoa husks protein with molecular weight of 22.8 kDa, 39.1 kDa and 52.7 kDa contained 17 kinds of amino acids, and the ratios of essential amino acids and hydrophobic amino acids to total amino acids were 35.17% and 28.48%, respectively. The hydrolysis ability of alkaline protease for quinoa husks protein was the highest, with degree of hydrolysis reached 13% at 120 min, and the peptides yield was 88.88%. The peptides produced by flavor protease showed high α-glucosidase inhibitory activity and Fe2+ chelating ability, which were 81.67% and 89.03% respectively; the inhibition rate of alkaline protease produced peptides against tyrosinase was 73.17%, while neutral protease produced peptides had strong scavenging ability to DPPH free radicals, which was 84.91%. This study showed that quinoa peptides had good biological activities, which could provide a theoretical basis for further development and utilization of quinoa husks.
林冰洁,薛鹏,荆金金,等. 不同蛋白酶制备藜麦麸皮多肽及其活性研究[J]. 食品与发酵工业, 2021, 47(3): 114-119.
LIN Bingjie,XUE Peng,JING Jinjin,et al. Activity of polypeptides from Chenopodiumquinoa husks prepared with different proteases[J]. Food and Fermentation Industries, 2021, 47(3): 114-119.
 VEGA-GALVEZ A,MIRANADA M,VERGARA J,et al.Nutrition facts and functional potential of quinoa (Chenopodium quinoa willd.),an ancient Andean grain:A review[J].Journal of the Science of Food and Agriculture,2010,90(15):2 541-2 547.  AYYASH M,JOHNSON S K,LIU S Q,et al.Cytotoxicity,antihypertensive,antidiabetic and antioxidant activities of solid-state fermented lupin,quinoa and wheat by Bifidobacterium species:In vitro investigations[J].LWT-Food Science and Technology,2018,95:295-302.  丁云双,曾亚文,闵康,等.藜麦功能成分综合研究与利用[J].生物技术进展,2015,5(5):340-346. DING Y S,ZENG Y W,MIN K,et al.Comprehensive research and utilization of functional components in quinoa[J].Current Biotechnology,2015,5(5):340-346.  范三红,田旭静,张锦华.藜麦糠抗氧化肽的超滤分离及其体外抗氧化活性[J].食品工业科技,2018,39(5):59-64. FAN S H,TIAN X J,ZHANG J H.The separation of the antioxidant peptide from quinoa chaff by ultrafiltration and its antioxidant activity in vitro[J].Science and Technology of Food Industry,2018,39(5):59-64.  NONGONIERMA A B,LE M S,DUBRULLE C,et al.Quinoa (Chenopodium quinoa Willd.) protein hydrolysates with in vitro dipeptidyl peptidase IV (DPP-IV) inhibitory and antioxidant properties[J].Journal of Cereal Science,2015,65:112-118.  叶凯,李小强,周金虎,等.响应面法酶解藜麦蛋白制备α-淀粉酶抑制肽的工艺研究[J].中国调味品,2019,44(12):6-11. YE K,LI X Q,ZHOU J H,et al.Study on enzymatic hydrolysis of quinoa protein for preparing α-amylase inhibitory peptide by response surface methodology[J].China Condiment,2019,44(12):6-11.  VILCACUNDO R,MIRALLES B,CARRILLO W,et al.In vitro chemopreventive properties of peptides released from quinoa (Chenopodium quinoa Willd.) protein under simulated gastrointestinal digestion[J].Food Research International,2018,105:403-411.  REN G X,ZHU Y,SHI Z,et al.Detection of lunasin in quinoa (Chenopodium quinoa Willd.) and the in vitro evaluation of its antioxidant and anti‐inflammatory activities[J].Journal of the Science of Food and Agriculture,2017,97(12):4 110-4 116.  ALUKO R E,MONU E.Functional and bioactive properties of quinoa seed protein hydrolysates[J].Journal of Food Science,2003,68(4):1 254-1 258.  赵雷,李晓娜,史龙龙,等.藜麦麸皮营养成分测定及其油脂的抗氧化活性研究[J].现代食品科技,2019,35(11):199-205;151. ZHAO L,LI X N,SHI L L,et al.Determination of the nutritional components in quinoa bran and the antioxidant activity of its derived oils[J].Modern Food Science and Technology,2019,35(11):199-205;151.  姜燕蓉,张亚飞,齐筱莹,等.等电点沉淀法提取牡蛎蛋白及其蛋白组成分析[J].渔业研究,2019,41(2):106-112. JIANG Y R,ZHANG Y F,et al.Isoelectric point precipitation method was used to extract oyster protein and analyze its composition[J].Journal of Fisheries Research,2019,41(2):106-112.  NISSEN J A.Enzymatic hydrolysis of food proteins[M].London:Elsevier Applied Science Publishers,1986.  沈佳奇.麦芽根多肽制备及其抑制α-葡萄糖苷酶活性的研究[D].广州:华南理工大学,2019. SHEN J Q.Preparation of malt root polypeptide and its inhibition on α-glucosidase activity[D].Guangzhou:South China University of Technology,2019.  MEDINA-MEZA I G,ALUWI N A,SAUNDERS S R,et al.Profiling of triterpenoid saponins from 28 quinoa varieties (Chenopodium quinoa Willd) grown in Washington State by GC-MS[J].Journal Agricultural Food Chemistry,2016,64(45):8 583-8 591.  DECKER E A,WELCH B.Role of ferritin as a lipid oxidation catalyst in muscle food[J].Journal of Agricultural & Food Chemistry,1990,38(3):674-677.  赵璇.洋葱蛋白及多肽的制备及其体外抗氧化活性评价[J].中国调味品,2020,45(2):112-115. ZHAO X.Preparation of onion protein and peptides and evaluation of their antioxidant activity in vitro[J].China Condiment,2020,45(2):112-115.  RUIZ G A,WU K X,MARTINUS V B,et al.Effect of extraction pH on heat-induced aggregation,gelation and microstructure of protein isolate from quinoa (Chenopodium quinoa Willd)[J].Food Chemistry,2016,209:203-210.  VALENZUELA C,ABUGOCH L,TAPIA C,et al.Effect of alkaline extraction on the structure of the protein of quinoa (Chenopodium quinoa Willd.) and its influence on film formation[J].International Journal of Food Science & Technology,2013,48(4):843-849.  CHEN H M,MURAMOTO K,YAMAUCHI F.Structural analysis of antioxidative peptides from soybean b-conglycinin[J].Journal of Agricultural and Food Chemistry,1995,43:574-578.  ADAMSON N J,REYNOLDS E C.Characterization of casein phosphopeptides prepared using alcalase:Determination of enzyme specificity[J].Enzyme and Microbial Technology,1996,19(3):202-207.  ZENG L,ZHANG G,LIAO Y,et al.Inhibitory mechanism of morin on α-glucosidase and its anti-glycation properties[J].Food & Function,2016,7(9):3 953-3 963.  INES K,OLFA T,DORRA G,et al.Antioxidant,antityrosinase and antibiofilm activities of synthesized peptides derived from Vicia faba protein hydrolysate:A powerful agents in cosmetic application[J].Industrial Crops & Products,2017,109:310-319.  GUO L,HOU H,LI B,et al.Preparation,isolation and identification of iron-chelating peptides derived from Alaska pollock skin[J].Process Biochemistry,2013,48(5-6):988-999.