Abstract: In this study, the effects of four strains of Lactobacillus paracasei LC2, LC3, LC38, and FJSWX33-L2 on constipation relief were evaluated, and the possible pathways of their differential effects were analyzed. Thirty-six Balb/c male mice were randomly divided into 6 groups. The normal group and the model group were intragastrically administered with 3% sucrose solution and the other groups were administered with L. paracasei for 14 days (109 CFU/ (d·each)). From the 15th day to the 30th day, except for the procedure same as the first 14 days, the suspension of loperamide was administered to establish a constipation model before gavage. The water content of the feces, the time to the first black stool and the rate of small bowel advancement were measured during the experiment; the levels of peptide tyrosine-tyrosine (PYY) and motilin in serum were determined; the levels of serotonin in the colon and neurotrophin-3 in the colon were measured; the transcription of Aqp4 and c-kit in the colon was detected; and the content of short-chain fatty acids (SCFAs) in the feces was also determined. All the four strains of L. paracasei showed the role of relieving constipation, while with differences in the pathway. LC2 could alleviate the constipation through the regulation on the level of PYY, NT-3, 5-HT, Aqp4, c-kit and SCFAs, whilst other three strains showed different ways to relieve constipation. It is indicated that L. paracasei has the potential to relieve constipation, while the effects on the constipation alleviation showed significant intra-species differences.
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