Abstract: In order to clarify the antibacterial effect of antagonistic Bacillus strains YBGJ1 and YBGJ2 on postharvest rot-causing fungi of Ziziphus jujube cv. Lingwu changzao and their preservation effect, these two antagonistic Bacillus strains were identified by morphological, physiological, biochemical and molecular biology methods, and their antibacterial and preservation effects were determined by in-dish confrontation culture and in vitro preservation test. The results showed that strain YBGJ1 and YBGJ2 were identified as Bacillus subtilis and Bacillus amyloliquefaciens. The mycelial inhibition rates of YBGJ1 and YBGJ2 culture filtrates for Botrytis cinerea were 95.83% and 95.79% respectively, and that for Alternaria alternata were 79.46% and 78.56% respectively. When the culture filtrate volume fractions of strains YBGJ1 and YBGJ2 were 50%, the spore germination inhibition rates for B. cinerea were 85.00% and 91.09% respectively, and that for A. alternata were 80.98% and 84.38% respectively. In the in vitro preservation test, the culture filtrate of strain YBGJ1 with volume fraction of 30% and that of strain YBGJ2 with volume fraction of 10% had the best preservation effects on Lingwu changzao. Compared with sterile water control, the decay rate was reduced by 70.83% and 77.08%, respectively, and the shelf life was extended by 15-20 days. Antagonistic Bacillus strains YBGJ1 and YBGJ2 are promising strains for fruits and vegetables preservation.
ZHOU T, SCHNEIDER K E, LI X. Development of biocontrol agents from food microbial isolates for controlling post-harvest peach brown rot caused by Monilinia fructicola[J]. International Journal of Food Microbiology, 2008, 126(1-2): 180-185.
LI J, YANG Q, ZHAO L. Purification and characterization of a novel antifungal protein from Bacillus subtilis strain B29[J]. Journal of Zhejiang University (Science B:An International Biomedicine & Biotechnology Journal), 2009, 10(4): 264-272.