Abstract: The concept of proteomics and its major research technical route were introduced,from the preparation of proteome in the sample,the two-dimensional polyacrylamide gel electrohporesis,to the identification of proteins by mass spectrum coupled with database search.The application of proteomics and its techniques in beer industry were summarized,and they were mainly used to distinguish barley varieties,and reveal the relationship between proteome and qualities of malt and beer.The research direction on the proteomics of beer and its raw materials was prospected.The research strategy would play more and more important role in the study on the quality of beer and its raw materials.