Abstract: In this experiment,a high-salt diet model was established in mice.The model mice were given pyroglutamic acid intervention to explore the effect of pyroglutamic acid on intestinal health and intestinal flora in mice with high-salt diet.Pathological analysis and morphological observation were performed on the small intestine of mice,followed by detection of intestinal inflammatory cytokines and albumin leakage in feces.Alpha diversity,beta diversity,and intestinal flora composition at the phylum and genus levels were analyzed by high-throughput sequencing.The results showed that pyroglutamic acid significantly alleviated the intestinal structural damage of mice caused by high-salt diet and reduced the secretion of inflammatory cytokines in small intestine.While pyroglutamic acid inhibited the significant increase in the abundance of some flora caused by high-salt diet,such as Bacteroides,Alistipes and other microorganisms.In addition,pyroglutamic acid also restored and increased the abundance of flora that had been reduced during high-salt diet,including Candidatus Arthromitus,Bifidobacterium,Lactobacillus and other probiotics.In summary,pyroglutamic acid has a significant beneficial effect on high-salt diet mice,which can stabilize the intestinal structure,reduce the level of intestinal inflammation,and improve the intestinal flora structure and abundance.
徐珒昭,汤梦琪,徐境含,等. 焦谷氨酸对高盐饮食小鼠肠道健康及肠道菌群的作用[J]. 食品与发酵工业, 2021, 47(2): 102-108.
XU Jinzhao,TANG Mengqi,XU Jinghan,et al. Effects of pyroglutamic acid on intestinal health and intestinal microflora in mice with high-salt diet[J]. Food and Fermentation Industries, 2021, 47(2): 102-108.
[1] XU A,MA J X,GUO X L,et al.Association of a province-wide intervention with salt intake and hypertension in Shandong province,China,2 011-2016[J].JAMA Internal Medicine,2020,180(6):877-886. [2] 陈卫,田培郡,张程程,等.肠道菌群与人体健康的研究热点与进展[J].中国食品学报,2017,17(2):1-9. CHEN W,TIAN P J,ZHANG C C,et al.New progress of research on gut microbiota and human health[J].Journal of Chinese Institute of Food Science and Technology,2017,17(2):1-9. [3] ZHAO X,YANG X X,ZHANG X L,et al.Dietary salt intake and coronary atherosclerosis in patients with prehypertension[J].Journal of Clinical Hypertension (Greenwich),2014,16(8):575-580. [4] YAN X F,JIN J J,SU X H,et al.Intestinal flora modulates blood pressure by regulating the synthesis of intestinal-derived corticosterone in high salt-induced hypertension[J].Circulation Research,2020,126(7):839-853. [5] FARHANA A K,KARINA A.Maternal elevated salt consumption and the development of autism spectrum disorder in the offspring[J].Journal of Neuroinflammation,2019,16(1):265. [6] BAI J,YU X J,LIU K L,et al.Tert-butylhydroquinone attenuates oxidative stress and inflammation in hypothalamic paraventricular nucleus in high salt-induced hypertension[J].Toxicolgy Letters,2017,281:1-9. [7] OMBRETTA M,SIMONA F,LUIGI M.Organic acids profile in tomato juice by HPLC with uv detection[J].Journal of Food Quality,2007,30(2):253-266. [8] MIROSLAVAš,SHERIF T S H,HAROON K,et al.A multi-biochemical and in silico study on anti-enzymatic actions of pyroglutamic acid against PDE-5,ACE,and urease using various analytical techniques:Unexplored pharmacological properties and cytotoxicity evaluation[J].Biomolecules,2019,9(9):392. [9] GANG F L,ZHU F,LI X T,et al.Synthesis and bioactivities evaluation of l-pyroglutamic acid analogues from natural product lead[J].Bioorganic &Medicinal Chemistry,2018,26(16):4 644-4 649. [10] YI L Z,SHI S T,WANG Y,et al.Serum metabolic profiling reveals altered metabolic pathways in patients with post-traumatic cognitive impairments[J].Scientific Reports,2016,6:21 320. [11] YOSHINARI O,IGARASHI K.Anti-diabetic effect of pyroglutamic acid in type 2 diabetic Goto-Kakizaki rats and KK-Ay mice[J].British Journal of Nutrition,2011,106(7):995-1 004. [12] FELIX S,MOLTZAU A J,RICHA B,et al.The resilience of the intestinal microbiota influences health and disease[J].Nature Reviews Microbiology,2017,15(10):630-638. [13] SPRAGUE A H,KHALIL R A.Inflammatory cytokines in vascular dysfunction and vascular disease[J].Biochemical Pharmacology,2009,78(6):539-552. [14] 李梦寒,王志勇,盛雪,等.基于16S rRNA技术分析α-乳白蛋白对大鼠肠道菌群的影响[J].食品科学,2020,41(6):155-162. LI M H,WANG Z Y,SHENG X,et al.Effect of alpha-Lactalbumin on intestinal flora in rats analyzed by 16S rRNA high-throughput sequencing[J].Food Science,2020,41(6):155-162. [15] MCDONALD D,VÁZQUEZ-BAEZA Y,KOSLICKI D,et al.Striped unifrac:Enabling microbiome analysis at unprecedented scale[J].Nature Methods,2018,15(11):847-848. [16] XIAO Z X,HU X J,ZHANG X M,et al.High salt diet accelerates the progression of murine lupus through dendritic cells via the p38 MAPK and STAT1 signaling pathways[J].Signal transduction and targeted therapy,2020,5(1):34. [17] WU C,YOSEF N,THALHAMER T,et al.Induction of pathogenic TH17 cells by inducible salt-sensing kinase SGK1[J].Nature,2013,496(7 446):513-517. [18] 黎砚书,董一飞,伍锡栋,等.高盐高蛋白饮食对Dah1/SS大鼠血压、肾脏和消化道病理变化的影响[J].实验动物科学,2014,31(6):11-16. LI Y S,DONG Y F,WU X D,et al.The effect of high salt and high protein diet on blood pressure and the pathological changes of kidney and gastrointestinal in Dah1/SS rats[J].Laboratory Animal Science,2014,31(6):11-16. [19] 李焱,高翔,王平贵,等.以贝壳为钙源的焦谷氨酸钙抗疲劳功效研究[J].中国海洋药物,2020,39(3):64-70. LI Y,GAO X,WANG P G,et al.Study on the anti-fatigue effect of calcium L-pyroglutamate with calcium derived from shell[J].Chinese Journal of Marine Drugs,2020,39(3):64-70. [20] FRICK J,AUTENRIETH I B.The gut microflora and its variety of roles in health and disease[J].Curr Top Microbiol Immunol,2013,358:273-289. [21] HAWKINS R A,SIMPSON I A,MOKASHI A,et al.Pyroglutamate stimulates Na+-dependent glutamate transport across the blood-brain barrier[J].FEBS Letters,2006,580(18):4 382-4 386. [22] WANG C,HUANG Z X,YU K Q,et al.High-salt diet has a certain impact on protein digestion and gut microbiota:A sequencing and proteome combined study[J].Frontiers in Microbiology,2017,8:1 838. [23] 訾雨歌,徐越,肖瀛,等.原花青素B2对D-半乳糖模型小鼠肠道菌群的影响[J].食品科学,2019,40(9):146-151. ZI Y G,XU Y,XIAO Y,et al.Effect of procyanidin B2 on intestinal microorganisms in D-galactose-induced aging mouse model[J].Food Science,2019,40(9):146-151. [24] 古俊,刘金彪,霍文哲.BBI阻断LPS对肠道上皮细胞间紧密连接蛋白的抑制作用[J].中国感染控制杂志,2018,17(3):185-190. GU J,LIU J B,HUO W Z.BBI blocks LPS-mediated inhibitory effect on tight junction protein of intestinal epithelial cells[J].Chinese Journal of Infection Control,2018,17(3):185-190. [25] 王宇,尹业师,王欣.肠道分节丝状菌(SFB)与宿主间相互作用关系研究进展[J].微生物学免疫学进展,2011,39(3):78-81. WANG Y,YIN Y S,WANG X.Research progress on the interaction between intestinal segmented filamentous bacteria (SFB) and the host[J].Progress in Microbiology and Immunology,2011,39(3):78-81. [26] LIAO N G,YIN Y S,SUN G C,et al.Colonization and distribution of segmented filamentous bacteria (SBF) in chicken gastrointestinal tract and their relationship with host immunity[J].FEMS Microbiology Ecology,2012,81(2):395-406. [27] SANDERS M E.Probiotics and microbiota composition[J].BMC Medicine,2016,14(1):82.