Synthesis of trehalose by Zygosaccharomyces mellis
ZHANG Minqian1, LIU Gongliang1*, FEI Yongtao1, BAI Weidong1, AI Lianzhong2, YU Jianshen3
1(College of Light Industry and Food Science, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China) 2(School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China) 3(Shanghai Jinfeng Wine Co.Ltd., Shanghai 200120, China)
Abstract: At present, research on the synthesis of trehalose with food grade microorganisms has attracted much attention. Since Zygosaccharomyces mellis LGL-2 can tolerate high glucose concentration and synthesize trehalose through stress metabolism, the effects of fermentation time, inoculation amount, culture temperature, initial concentration of glucose and rotational speed on trehalose production by LGL-2 were studied. The optimal fermentation conditions were determined by response surface methodology. The optimal conditions were determined as follows: 96 h, 10% inoculation size, 300 g/L initial glucose concentration, 24 ℃ and 180 r/min. Under the optimized conditions, the yield of trehalose was 92.32 mg/g dry yeast, which was 29.34% higher than that before optimization. The results provide reference for the application of Z. mellis and the biosynthesis of trehalose.
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2021, Vol. 47 
