Abstract: African swine fever (ASF) is an acute, hemorrhagic and severe infectious disease caused by African swine fever virus (ASFV) which infect domestic pigs and wild boars. The continued spread of ASF not only affects the meat supply of residents, but also the safety of global meat supplied. Therefore, the establishment of sensitive and specific ASFV detection method has become an important issue in food safety. In this work, we reported a fluorescence resonance energy transfer (FRET) DNA biosensor based on quantum dots (QDs) and gold nanoparticles (AuNPs) for ASFV specific gene detection. In the absence of target DNA, ss-DNA-QDs (Probe 1) will hybridize with ss'-DNA-AuNPs (Probe 2) making the donor (QDs) and the acceptor (AuNPs) close to each other and trigger FRET effect, and the fluorescence of QDs was quenched by AuNPs. However, in the presence of target DNA, the target DNA competed with Probe 2 to bind Probe 1, which resulted in the destruction of FRET and recovery of the fluorescence of QDs. The developed biosensor in here could achieve rapid detection of ASFV target DNA with the detection limit of 0.72 μmol/L in 1.25 h. Which was verified in pork, ham sausage, and pork dumplings with recoveries from 82.00% to 108.00% and variation coefficients of 0.02%-0.15%. This study proposed a new, simple and rapid method to detect ASFV gene fragment in food. In addition, this strategy can be used to other DNA, virus or protein sensing applications.
乐莉,张亚青,宋尔群,等. 一种荧光DNA生物传感器用于食品中非洲猪瘟病毒的检测[J]. 食品与发酵工业, 2021, 47(9): 268-274.
YUE Li,ZHANG Yaqing,SONG Erqun,et al. A fluorescent DNA biosensor for the detection of African swine fever virus in foods[J]. Food and Fermentation Industries, 2021, 47(9): 268-274.
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