Effect of exogenous gene expression on sugar alcohol metabolism of Yarrowia lipolytica
ZHOU Yunqi1, LI Hongjiao1, LI Kewen2, GUO Bei3, PEI Jiangsen1*
1(China National Research Institute of Food and Fermentation Industries, Beijing 100015, China) 2(Bao Ling Bao Biology Company Limited, Yucheng 251200, China) 3(College of Bioscience and Resources Environment, Beijing University of Agriculture, Beijing 102206, China)
Abstract: Yarrowia lipolytica is a widely used eukaryotic expression host and a major strain for erythritol fermentation in China. In this research, a putative gene with sugar phosphorylation function cloned from Zygoascus hellenicus was constructed into an expression vector and transformed into Y. lipolytica. The effect of the recombinant strain on substrate utilization and conversion related to erythritol synthesis was investigated. The results showed that in the shake flask fermentation, different assimilation of erythritol and ribose was observed in the recombinant Y. lipolytica compared with the original strain. With glucose and erythritol/ribose in the medium, the assimilation ability of the recombinant strain was at least twice that of the original strain. This functional gene was a new gene with the ability to regulate erythritol synthesis. The treatment experiment of erythritol waste liquor showed that the recombinant strain could assimilate various sugar alcohols for cell growth compared with the control strain, and change the composition of the crystallization waste liquor to reduce the viscosity and maltose concentration, providing a new solution for the comprehensive utilization of industrial waste liquor.
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