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食品与发酵工业  2021, Vol. 47 Issue (21): 275-281    DOI: 10.13995/j.cnki.11-1802/ts.027130
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李林波*, 王宝石, 张明霞, 杨天佑*, 李志刚
(河南科技学院 生命科技学院,现代生物育种河南省协同创新中心,河南 新乡,453003)
Strategical analysis of high-efficient synthesis of isomalto-oligosaccharides by α-glucosidase
LI Linbo*, WANG Baoshi, ZHANG Mingxia, YANG Tianyou*, LI Zhigang
(School of Life Science and Technology, Henan Collaborative Innovation Center in Modern Biological Breeding, Henan Institute of Science and Technology, Xinxiang 453003, China)
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摘要 α-葡萄糖苷酶作为工业化生产低聚异麦芽糖(isomaltooligosaccharides,IMOs)的关键酶制剂,因其发挥的提高催化效率和降低生产成本的重要作用,而受到学者的广泛关注。文章综述了α-葡萄糖苷酶的来源、性质、分类和催化机制,分析了α-葡萄糖苷酶高效合成IMOs的策略,并对工业化高效生产IMOs进行了展望,旨在为高效清洁生产IMOs提供参考。
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关键词:  α-葡萄糖苷酶  低聚异麦芽糖  异源表达  高效合成  酵母表面展示技术    
Abstract: α-glucosidase is a key enzyme preparation for industrial production of isomalto-oligosaccharides (IMOs), and it plays an important role in improving the catalytic efficiency and reducing the production cost, which has been widely concerned by scholars. The sources, properties, classification and catalytic mechanism of α-glucosidase were reviewed, and the strategy for high-efficient synthesis of IMOs by α-glucosidase was analyzed. The prospect of industrial and high-efficient production of IMOs was also discussed, aiming to provide a reference for high-efficient and clean production of IMOs.
Key words:  α-glucosidase    isomalto-oligosaccharides    heterologous expression    high-efficient synthesis    yeast surface display technology
收稿日期:  2021-02-26      修回日期:  2021-03-26           出版日期:  2021-11-15      发布日期:  2021-11-30      期的出版日期:  2021-11-15
基金资助: 河南科技学院攀登计划(201010916007);河南省重点研发与推广专项(科技攻关)(192102210193)
作者简介:  博士,讲师(李林波博士和杨天佑副教授为共同通讯作者,;
李林波,王宝石,张明霞,等. α-葡萄糖苷酶高效合成低聚异麦芽糖的策略分析[J]. 食品与发酵工业, 2021, 47(21): 275-281.
LI Linbo,WANG Baoshi,ZHANG Mingxia,et al. Strategical analysis of high-efficient synthesis of isomalto-oligosaccharides by α-glucosidase[J]. Food and Fermentation Industries, 2021, 47(21): 275-281.
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