以甘蔗渣残渣、聚丙烯微米纤维膜和棉纤维分别作为载体固定琥珀酸放线杆菌进行丁二酸的发酵生产，通过优化载体使用量获得较高的丁二酸产量并进行反复分批发酵以研究载体重复使用性能。研究发现聚丙烯微米纤维膜产丁二酸相对最优，且相对使用量最少，而棉纤维材料重复使用稳定性最优。以棉纤维材料构建转动式纤维床反应器，研究不同pH调节剂对反应器运行效率的影响，发现以MgCO3作为pH调节剂时，丁二酸浓度分别比以NH3×H2O-Na2CO3和KOH-K2CO3作为调节剂时提高32%与20%。在反应器中采用反复补料分批发酵的操作方式，平均每35 h一个批次，发酵运行175 h，丁二酸平均浓度为87.8 g/L，平均生产强度为2.51 g/L×h，平均转化率为0.86 g/g。结果表明，构建的纤维床反应器有利于实现丁二酸的高浓度、高生产强度和高转化率。

In this paper, sugarcane bagasse residue, polypropylene microfiber membrane and cotton cloth were adopted as supports to immobilizeActinobacillussuccinogenesfor the fermentative production of succinic acid. The usage of supports was optimized to improve the yield of succinic acid and repeated batch fermentation was conducted to study the reusable stability of supports. Polypropylene microfiber membrane performed best in the production of succinic acid while cotton cloth performed best in the reusable stability test. As a result, cotton cloth was used in the construction of a fibrous-bed bioreactor. In the study of the effect of pH regulator on bioreactor efficiency, there were 32% and 20% increase in succinic acid concentration when using MgCO3as the pH regulator compared with that using NH3×H2O-Na2CO3and KOH-K2CO3. Repeated fed-batch fermentation was operated in the bioreactor with the average batch time of 35 h and the total operation time of 175 h. Results showed that the average concentration, productivity and yield of succinic acid were87.8 g/L, 2.51 g/L×h and 0.86 g/g, respectively. Results showed that the fibrous-bed bioreactor reported in this paper helped to achieve high concentration, high productivity and high yield of succinic acid.