红曲菌（Monascusspp.）是我国具有传统特色的药食两用微生物资源。它的主要代谢产物包括红曲色素（Monascuspigments，MPs）及莫纳可林K（monacolin K, MK）等。前期研究表明，甘油可促进红曲菌产MK和MPs。以高产MK和MPs但不产桔霉素的丛毛红曲菌（Monascus pilosus）MS-1为实验菌株，对比分析不同来源粗甘油和分析纯甘油对红曲菌生长及产MK和MPs的影响。结果表明：在固态发酵培养基中添加分析纯甘油可促进红曲菌产MK及MPs。当分析纯甘油添加量分别达到6%和3%时，MK和MPs的产量可达到最高，且MK和MPs的产量分别是对照的1.57倍和1.53倍。当添加6%的纯度为90%的脂肪醇粗甘油时，MK及MPs的产量可达到最高（p<0.05），且其同系物的种类均多于对照及添加分析纯甘油的样品。在液态发酵中，添加40 g/L的不同来源粗甘油及分析纯甘油后，均可显著降低发酵液的pH并显著提高生物量（p<0.05）。当添加90%生物柴油粗甘油和90%脂肪醇粗甘油时，MK及MPs的产量均显著增加且达到最高（p<0.05），分别是对照的11.57倍和11.08倍。MK及MPs的同系物种类相比对照均有所增加。粗甘油在MK及MPs的生产中有广阔的应用前景。

Monascusspp. is a traditional Chinese medicinal and edible microbial resource. Its main metabolites includingMonascuspigment (MPs), monacolin K (MK), etc. The previous results indicated that glycerol could improve MK and MPs production byMonascus. AMonascusstrain,Monascus pilosusMS-1, which could produce high yields of MK and MPs but non citrinin was used. And the effects of crude glycerol and analytical grade glycerol on the growth and production of MK and MPs by MS-1 were carried out. The results showed that analytical grade glycerol could improve MK and MPs production in solid-state fermentation by MS-1. The most suitable analytical grade glycerol concertations for MK and MPs produciton in solid-state fermentation was 6% and 3%, respectively, and the yields of MK and MPs was 1.57 and 1.53 folds to that of control, respectively. The highest yields of MK and MPs were obtained when 6% crude glycerol from fatty alcohol with 90% purity was used and the homologues were also more than that of control and which adding analytical grade glycerol in solid-state fermentation (p<0.05). In liquid fermentation, pH values of fermentation broths and biomass were significantly decreased and increased, respectively, when crude glycerol and analytical grade glycerol with concentration of 40 g/L were used (p<0.05). MK and MPs production were significantly enhanced when crude glycerol and analytical grade glycerol were used (p<0.05). MK and MPs yields were 11.57 and 11.08 folds to that of control when crude glycerol from biodiesel with 90% purity and crude glycerol from fatty alcohol with 90% purity were used, respectively. Moreover, the species of MK and MPs were increased compared to that of control. Crude glycerol possess huge potential use for MK or MPs production.