本研究从短乳杆菌、副干酪乳杆菌、植物乳杆菌、嗜酸乳杆菌、鼠李糖乳杆菌等乳酸菌中筛选出产乙醛脱氢酶的植物乳杆菌L. plantarumFCJX 102和嗜酸乳杆菌L.acidophiluFCJX 104。在单因素实验的基础上，以山楂葛根比、菌种比、发酵时间为影响因子，对产乙醛脱氢酶发酵条件进行正交实验优化。实验结果优化后在培养基中山楂葛根比为1:2，菌种L. plantarumFCJX 102与L. acidophiluFCJX 104比为2:1时，在37℃下培养16h，获得的乙醛脱氢酶活力最高可达16.083U/g。

L.plantarumFCJX 102 andL. acidophiluFCJX 104 producing acetaldehyde dehydrogenase were selected from five kinds of lactic acid bacteria including Lactobacillus brevis, Lactobacillus paracasei, Lactobacillus plantarum, Lactobacillus acidophilus and Lactobacillus rhamnosus. On the basis of single factor experiment, the fermentation conditions of hawthorn puerarin juice producing aldehyde dehydrogenase were studied by single factor and orthogonal optimization, hawthorn pueraria ratio, strain ratio and fermentation time as impact factors. Experimental results showed that when the rate of hawthorn and puerarinin the medium was 1:2 and the amount ratio ofL. plantarumFCJX 102 andL. acidophilu FCJX104 was 2:1, and cultured at 37 for 16,the highest enzyme activity of acetaldehyde dehydrogenase was obtained after optimization was 16.083U / g.