花椒麻味物质分子印迹固相萃取柱的制备及其应用 

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  • 1 (西南大学食品科学学院,重庆,400715) 2 (重庆市农产品加工及贮藏重点实验室,重庆,400715)3(西南大学园艺园林学院,重庆,400715)

网络出版日期: 2018-01-03

The preparation and application of the Chinese prickly ash’s molecularly imprinted solid-phase extraction

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  • 1(College of Food Science, Southwest University, Chongqing, 400715) 2(Laboratory of Quality & Safety Risk Assessment for Agro-products on Storage and Preservation (Chongqing), Ministry of Agriculture, 400715) 3(College of Gardening, Southwest University, Chongqing, 400715)

Online published: 2018-01-03

摘要

以α-(羟基)-山椒素分子结构类似物为模板分子、2-乙烯基吡啶(2-Vinylpyridine ,2-Vpy)为功能单体、二甲基丙烯酸乙二醇酯(Ethyleneglycol dimethacrylate, EDMA)为交联剂按1:4:20的摩尔比合成了花椒麻味物质硅胶表面分子印迹聚合物,并以合成的硅胶表面分子印迹聚合物为固相萃取柱填料,制备了花椒麻味物质分子印迹固相萃取柱,确定了该固相萃取柱的最佳上样溶剂、淋洗溶剂及洗脱溶剂,用此固相萃取柱对花椒油树脂中的花椒麻味物质进行分离纯化,结果表明,该固相萃取柱对花椒麻味物质表现出优异的特异性吸附效果,可以分离纯化出相对纯度为93.66 %的花椒麻味物质。

本文引用格式

陈晓龙, 李耀, 陈光静, 等 . 花椒麻味物质分子印迹固相萃取柱的制备及其应用 [J]. 食品与发酵工业, 2017 , 43(12) : 99 -107 . DOI: 10.13995/j.cnki.11-1802/ts.014711

Abstract

This study synthesized the molecularly imprinted polymers by using the molecular structure analogues of α-(OH)-sanshool as the template molecule, 2-Vpy as functional monomer, EDMA as crosslinking agent at the mole ratio of 1:4:20, and then the molecularly imprinted solid-phase extraction (MISPE) was prepared. The optimal sample solvent, drip washing solvent and elution solvent were selected out and the SMIP was used to separate and purify the numb taste components in Zanthoxylum oleoresin, the results show that the SMIP had the especially specific adsorption to the numb taste components which the relative purity of numb taste components can add to 93.66%.
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