Flavors
change of mulberry wine which was fermented by
Saccharomyces cerevisiae JM037 posessing γ-aminobutyric acid(GABA) production capacity were analyzed during fermentation process. The
changes of organic acid and volatile compounds were evaluated by high performance
liquid chromatography (HPLC) and head-space solid phase micro-extraction
associated with gas chromatography-mass spectrometry (HS-SPME/GC-MS),
respectively. Meanwhile, the production of GABA in mulberry
wine was measured. The results showed that 7 kinds of organic acids were
detected, and the content of them were firstly increased and then decreased,
totally;
54 volatile compounds were identified in mulberry wine, including
19 esters, 16 acohol, 8 aldehydes, 3 ketones, 3 phenols, 4 others., and the relative
content of volatile compounds from great to small was as follows: alcohols,
esters, ketones, aldehydes and phenols. Moreover, the kinds and relative
content of volatile compounds were changed during fermentation process. Finally
the principal component analysis (PCA) demonstrated that aldehydes,
ketones and phenols of mulberry provided the precursor substance for other
flavor compounds of the fermented mulberry wine. Furthermore, the concentration
of GABA in mulberry wine reached to 1 180 mg/L.