建立超高效合相色谱测定食品中游离氨基酸的方法。选取Acquity UPC2Torus Diol（3.0 mm×100 mm, 1.7 μm）色谱柱，流动相由A（超临界二氧化碳）和B（甲醇:乙腈=1:1）组成，2.5 mmol/L乙酸铵作为改性剂，流速2.0 mL/min，紫外检测器波长280 nm，补偿范围330~430 nm，背压1800 psi。结果表明：该方法具有良好的线性（R2= 0.9990~0.9998），分离度高于1.22，检出限和定量限范围分别为23~57 ng/L和70~178 ng/L，线性范围为20~400 μg/mL，加标回收率在85.6%-101.2%之间。该方法被成功用于火龙果酒样品中游离氨基酸含量的测定，也为分析其它食品基质中游离氨基酸分布情况提供了新的方法。

A new method based on ultra performance convergence chromatography (UPC2) was developed for analysis of free amino acids in fruit wine. They were separated on an Acquity UPC2Torus Diol (3.0 ×100mm, 1.7 μm) with a mobile phase consisted of supercritical CO2and methanol: acetonitrile (1: 1 v/v, with 2.5 mmol/L ammonium acetate as mobile phase modifier), the flow rate is 2.0 mL/min and DAD detection was set at 280 nm with a compensation range of 330-430 nm, back pressure is 1800 psi. The results showed good linearity (R2= 0.9990-0.9998) and high resolution higher than 1.22. The limits of detection (LOD) and quantification (LOQ) ranged from 23-57 ng/L and 70-178 ng/L, respectively. The linear range was between 20 and 400 μg/mL. The spiked recoveries were ranged from 85.6% to 101.12%. It was applied for the free amino acids analysis of selected pitaya wine, which could also provide a new method for the analysis of free amino acid distribution in other food matrix from different sources.