超高效合相色谱法测定火龙果果酒中游离氨基酸的含量

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  • (1.中国热带农业科学院农产品加工研究所,农业部农产品加工质量安全风险评估实验室,广东 湛江 524001;2.中国热带农业科学院农产品加工研究所,广东 湛江 524001;3.华中农业大学食品科技学院,湖北 武汉 430070;4.铜仁市食品药品检验所,贵州 铜仁 554300)

网络出版日期: 2018-01-08

基金资助

中国热带农业科学院基本科研业务费专项资金(1630122017021),湛江市科技计划项目(2016A03012),农业部公益性行业(农业)科研专项(201303077)

Determination of free amino acids in pitaya wine by ultra performance convergence chromatography

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  • (1. Agricultural Products Processing Research Institute, Chinese Academy of Tropical Agricultural Sciences, Laboratory of Quality and Safety Risk Assessment on Agro-products Processing, Ministry of Agriculture, Zhanjiang 524001, China; 2. Agricultural Products Processing Research Institute, Chinese Academy of Tropical Agricultural Sciences, Zhanjiang 524001, China; 3. College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China; 4. Tongren Institute for Food and Drug Control, Tongren 554300, China)

Online published: 2018-01-08

摘要

建立超高效合相色谱测定食品中游离氨基酸的方法。选取Acquity UPC2Torus Diol(3.0 mm×100 mm, 1.7 μm)色谱柱,流动相由A(超临界二氧化碳)和B(甲醇:乙腈=1:1)组成,2.5 mmol/L乙酸铵作为改性剂,流速2.0 mL/min,紫外检测器波长280 nm,补偿范围330~430 nm,背压1800 psi。结果表明:该方法具有良好的线性(R2= 0.9990~0.9998),分离度高于1.22,检出限和定量限范围分别为23~57 ng/L和70~178 ng/L,线性范围为20~400 μg/mL,加标回收率在85.6%-101.2%之间。该方法被成功用于火龙果酒样品中游离氨基酸含量的测定,也为分析其它食品基质中游离氨基酸分布情况提供了新的方法。

本文引用格式

齐宁利 , 龚霄 , 马丽娜 , 等 . 超高效合相色谱法测定火龙果果酒中游离氨基酸的含量[J]. 食品与发酵工业, 2017 , 43(12) : 199 -204 . DOI: 10.13995/j.cnki.11-1802/ts.015034

Abstract

A new method based on ultra performance convergence chromatography (UPC2) was developed for analysis of free amino acids in fruit wine. They were separated on an Acquity UPC2Torus Diol (3.0 ×100mm, 1.7 μm) with a mobile phase consisted of supercritical CO2and methanol: acetonitrile (1: 1 v/v, with 2.5 mmol/L ammonium acetate as mobile phase modifier), the flow rate is 2.0 mL/min and DAD detection was set at 280 nm with a compensation range of 330-430 nm, back pressure is 1800 psi. The results showed good linearity (R2= 0.9990-0.9998) and high resolution higher than 1.22. The limits of detection (LOD) and quantification (LOQ) ranged from 23-57 ng/L and 70-178 ng/L, respectively. The linear range was between 20 and 400 μg/mL. The spiked recoveries were ranged from 85.6% to 101.12%. It was applied for the free amino acids analysis of selected pitaya wine, which could also provide a new method for the analysis of free amino acid distribution in other food matrix from different sources.
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